基因敲除在工业微生物育种方面的应用  

Gene knockout and its applications in industrial microbe breeding

作　　者：宋安东[1] 张沙沙[1] 王风芹[1] 谢慧[1] 田原[1] 

机构地区：[1]河南农业大学生命科学学院,郑州450002

出　　处：《生物学杂志》2011年第6期68-72,共5页Journal of Biology

基　　金：国家农业成果转化资金项目(No.2006GB2D000173);河南省科技攻关重点项目(No.092102210110);河南省高等学校青年骨干教师资助计划

摘　　要：微生物育种技术的发展先后经历了自然选育、诱变育种、杂交育种、代谢控制育种和基因工程育种5个阶段,其中基因工程育种技术中的基因敲除技术具有定位性强、经修饰和改造的基因能够随染色体DNA的复制而稳定地复制的特点,使人们可以有目的地去改造生物的遗传物质,从而达到微生物育种的目的,受到人们的广泛关注。就基因敲除技术的几种主要方法及其在改良工业生产菌株中的应用作简要介绍。The development of microbe breeding technology has gone through five stages, including natural selecting breeding, mutation breeding, hybrid breeding, control of metabolic breeding and genetic engineering breeding. Gene knockout technology has .characteristic of efficient positioning, modified and transformation of genes stable reproduction with the chromosomal DNA replication in genetic engineering breeding, so people can intentionally transform the genetic material of biology, so as to achieve the purpose of microbial breeding. Gene knockout technology is being paid more and more attention by people. In this paper, several main methods of gene knockout technology and its improved application in industrial production strains were introduced.

关 键 词：工业微生物育种 基因敲除 同源重组 

分 类 号：Q784[生物学—分子生物学]