通过IFN-γ影响肿瘤相关巨噬细胞抑制胆囊癌血管发生的实验研究  

Responsiveness of tumor-associated macrophages to IFN-γ blocks angiogenesis of gallbladder cancer

作　　者：孙涛[1] 张明宇[1] 程颖[1] 葛春林[1] 

机构地区：[1]中国医科大学附属第一医院,沈阳110001

出　　处：《山东医药》2011年第48期25-27,119,共4页Shandong Medical Journal

基　　金：辽宁省科技计划课题(2009225010-19)

摘　　要：目的研究IFN-γ影响胆囊癌中肿瘤相关巨噬细胞(TAM)促血管发生的能力。方法通过接种人胆囊癌细胞株GBC-SD构建人胆囊癌裸鼠皮下肿瘤模型,给予肿瘤内注射IFN-γ处理,通过免疫组化法计数浸润的单核—巨噬细胞、TAM和微血管密度(MVD),并计算TAM分化率;通过ELISA法检测肿瘤内小鼠血管内皮生长因子(VEGF)的分泌情况。结果实验组肿瘤中浸润的单核—巨噬细胞高于对照组(P<0.01);TAM分化百分率,鼠源性VEGF和MVD均低于对照组(P<0.05或<0.01)。结论 IFN-γ能够抑制TAM的分化形成,降低胆囊癌中VEGF的浓度,减少胆囊癌的血管发生,从而抑制胆囊癌进展。Objective To investigate the effects of IFN-γ on tumor-associated macrophages （TAM） ability to pro-angiogenesis in gallbladder cancer. Methods We buihed gallbladder cancer model by inoculating subcutaneously the human gallbladder cancer cell line （GBC-SD） to BALB/C nude mice, and injected the IFN-γ of murine intratumorally. Immunohistochemistry was performed to count the monocyte-macrophage, TAM and microvessel density （MVD）. ELLSA was performed to assay the vascular endothelial growth factor （VEGF） secretion of nude mice. Results In experimental group, monocyte-macrophage was significantly higher than that in control group （P 〈0.01 ）, and TAM differentiation percentage, mice VEGF and MVD were significantly lower than those in control group （P 〈 0.05 or 〈 0.01 ）. Conclusion IFN-γ can inhibit the tumor growth by inhibiting the differentiation from monocyte to TAM, reducing the concentration of VEGF and angiogenesis in the gallbladder cancer.

关 键 词：胆囊肿瘤 肿瘤相关巨噬细胞 干扰素Γ 免疫治疗 

分 类 号：R735.8[医药卫生—肿瘤]