先天性心脏病并肺动脉高压患者肺组织中MMP-1、TIMP-1的表达研究  

Expression of MMP-1 and TIMP-1 in lung tissues of the CHD with pulmonary hypertension

作　　者：兰碧洋[1,2] 覃家锦[1] 冼磊[1] 赵文[1] 陈小三[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西民族医院

出　　处：《山东医药》2011年第48期39-41,119,共4页Shandong Medical Journal

基　　金：广西科学基金资助项目(桂科自0640087)

摘　　要：目的探讨基质金属蛋白酶1(MMP-1)及其抑制因子1(TIMP-1)在先天性心脏病(CHD)合并肺动脉高压(PH)发病机制中的可能作用。方法光镜下观察60例CHD患者肺小动脉形态,按照Heath-Edwards PH分级法分组,CHD无PH患者14例为对照组,CHD合并PHⅠ、Ⅱ、Ⅲ级共46例为实验组。采用免疫组化SP法和RT-PCR法分别测定患者肺组织MMP-1、TIMP-1蛋白及mRNA的表达情况。结果 MMP-1 mRNA、TIMP-1 mRNA、TIMP-1/MMP-1、MMP-1蛋白、TIMP-1蛋白在对照组及各实验组间存在差异,且均与PH病理分级正相关(P<0.05或<0.01)。结论 MMP-1、TIMP-1表达增高和二者比例失衡与CHD并发PH的形成与发展有关,可能是PH患者肺血管重构的发病机制之一。Objective To investigate the effects of matrix metalloproteinase 1 （ MMP-1 ） and tissue inhibitor of matrix metalloproteinase 1 （TIMP-1） in congenital heart disease （CHD） with pulmonary hypertension （PH）. Methods Sixty patients with CHD were observed of pulmonary artery morphology and divided into 4 groups according to Heath-Edwards pulmonary vessel pathologic grading： control gro, p （without PH, n = 14） and PH Ⅰ , Ⅱ and Ⅲ combined with CHD groups （n =46）. RT-PCR and immunohistochemical SP method were employed to detect the mRNA and proteins of MMP- 1, TIMP-1 in the lung tissues of these patients. Results There were differences in the expression of MMP-1 mRNA, TIMP-1 mRNA, TIMP-1/MMP-1, proteins of MMP-1 and TIMP-1 in control group and experimental groups. There was sig- nificantly positive correlation between above-mentioned index and the grade of PH. Conclusions Increased levels and imbalance of MMP-1/TIMP-1 associate with the development and acceleration of PH in CHD. They may play a role in the pathogenesis of pulmonary vascular remodeling.

关 键 词：基质金属蛋白酶1 基质金属蛋白酶组织抑制因子1 肺动脉高压 先天性心脏病 

分 类 号：R541.1[医药卫生—心血管疾病]