哮喘患儿诱导痰中TGF-β_1、IL-5与肺功能相关性的研究  

To study of the correlation between expressing of TGF-β_1,IL-5 level in induced sputum and pulmonary function of children with actue asthma

作　　者：陈强[1] 李莉[1] 刘建梅[1] 柯江维[2] 邹音[3] 

机构地区：[1]江西省儿童医院呼吸科,南昌330006 [2]江西省儿童医院检验科,南昌330006 [3]江西省儿童医院病理科,南昌330006

出　　处：《江西医药》2011年第11期988-991,共4页Jiangxi Medical Journal

基　　金：江西省科技厅社会发展攻关项目(200405)

摘　　要：目的探讨哮喘患儿诱导痰中TGF-β1表达、IL-5水平及E0S计数与肺功能的相关性。方法选取30例5-15岁支气管哮喘急性发作患儿(哮喘组)、该组30例经吸入糖皮质激素治疗6个月-1年进入哮喘缓解期的患儿(缓解组),30例5-15岁健康儿童(对照组),对3组病人测定诱导痰中TGF-β1表达、IL-5水平及E0S计数,3组病人均测定肺功能FEV1等值。结果(1)哮喘组TGF-β1阳性细胞表达率与缓解组比较,P<0.01;哮喘组IL-5、E0S与对照组、缓解组比较,P均<0.01;(2)哮喘组FEV1%pre、FEF25-75%均低于对照组及缓解组,P<0.05;缓解组FEV1%pre与对照组比较,差异无统计学意义;缓解组FEF25-75%低于对照组,P<0.05;(3)哮喘组痰液中TGF-β1阳性细胞表达率与IL-5水平、EOS%呈正相关:r=0.38 P<0.05,r=0.66,P<0.01;与肺功能FEV1%pre、FEF25-50呈负相关:r=-0.68,P<0.01,r=-0.555,P<0.05。结论 TGF-β1、IL-5、EOS在哮喘患儿诱导痰中有过度表达,这些指标与肺功能有很好的相关性,可作为气道慢性炎症的一个标示物用来监测哮喘。Objective To study the correlation between expressing of TGF-J31,IL-5 level,EOS count in induced sputum and pul-monary function of children with actue asthma.Methods Selection of 30 cases of 5 to 15 years old the asthma patients （asthma group）, they inhaled corticosteroids for six months - 1 years into remission of asthma patients （ease group）, 30 cases of 5 to 15 years old healthy children were served as the control group, Expressing of TGF-β1,the IL- 5 level,EOS count in induced sputum was deter-mined, The forced expiratory ratio（FEVL） were measured on the three groups of patients.Results （1）The positive expression of TGF- β1 in inflammatory cells in induced sputum were higher in asthma group than in ease group P 〈 0.01 ;The expression of IL-5 and EOS count were higher in asthma group than in control group and ease group, P〈0.01 respectively.（2）Asthma group FEV1% pre, FEF25-75% were lower than those of the control group and ease group,P〈0.05 respectively; the difference between FEVl%pre of control east＇ group and control group were no statistically significant;The FEF25%-75% were lower in ease group than in control group,P〈0.05. （3）The positive expression of TGF-β1 in inflammatory cells in induced sputum of asthma group were positively correlated with IL-5 level and EOS count r=0.38, P〈0.05, r=0.66, P〈0.01, and negative correlation with FEV 1%pre,FEF25-50%, r=-0.68, P〈0.01, r=-0.555, P〈0.05.Conclusion The over-expression of TGF-I3j,IL-5,EOS count in induced sputum of asthma patients,have good correlaton with pulmonary function ,can be used as a chronic inflammatory airway markers to monitor asthma.

关 键 词：哮喘 儿童 转化生长因子-Β1 第一秒用力呼气容积 用力呼气中段流量 白介素-5 诱导痰 

分 类 号：R725.6[医药卫生—儿科]