羔羊超数排卵及体外受精技术研究  

The effect of different treatments on superovulation and in vitro fertilization(IVF) efficiency

作　　者：吴海凤 李发弟[2] 常卫华[3] 马友记[2] 张志强[3] 刁睿鑫[3] 张勇[1,3] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃农业大学动物科学技术学院,甘肃兰州730070 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2011年第6期1-5,16,共6页Journal of Gansu Agricultural University

基　　金：科技部国家科技支撑计划(2008BADB2B10-7);农业部转基因生物新品种培育重大专项(2008ZX08008-003,2009ZX08008-008B)

摘　　要：用FSH+PMSG和FSH+LH 2种处理方法,对8只6周龄的高山细毛羊进行超数排卵试验,4只作对照组,并对采集到的卵子进行体外受精,探索羔羊超数排卵的可行性及体外受精的效果.结果表明:2组处理共获得发育卵泡583个,回收卵母细胞255枚.卵母细胞经体外培养24h后与上游法获能的精子进行体外受精,48h后2组处理的卵裂率分别为11.4%和22.7%.采用6周龄的羔羊进行超数排卵是完全可行的,而且成熟卵母细胞经体外受精后FSH+LH的处理组卵裂率明显高于FSH+PMSG处理组.In order to explore the feasibility of superovulation in lamb and the in vitro fertilization of oocytes obtained from superovulation,8 lambs of Gansu Alpine sheep aged 6-weeks-old were superovulated by 2 different treatments with FSH＋LH and FSH＋PMSG,and four lambs were used as control.The results indicated that the number of developed follicles were 583 and oocytes recovered were 255 after treatments.The oocytes were fertilized in vitro with the sperm capacitated by swim up after being in vitro matured for 24 hours.The cleavage ratio after 48 h were 11.4% and 22.7% respectively.6-week-old lamb for superovulation was entirely feasible,and the cleavage ratio of the treatment with FSH ＋ LH had an advantage over the method of FSH＋PMSG.

关 键 词：羔羊 超数排卵 体外受精 

分 类 号：S826.8[农业科学—畜牧学]