2株拮抗菌的抑菌作用及产酶能力初探  

Inhibitory effects of two antagonistic bacterial strains and theirs producing extracellular enzymes ability in vitro

作　　者：田恩琴[1] 常永义[2] 朱建兰[1] 靳小刚[3] 雷成军[1] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃省静宁县林业局,甘肃静宁743400

出　　处：《甘肃农业大学学报》2011年第6期41-45,51,共6页Journal of Gansu Agricultural University

基　　金：现代农业产业技术体系专项资金资助(Supported by China Agriculture Research system(30))

摘　　要：利用从葡萄果穗附生微生物区系分离获得的拮抗细菌BHG和BTZ,对葡萄灰霉病菌(Botrytis cinerea)进行抑菌试验.结果表明:BHG和BTZ与葡萄灰霉病菌对峙培养5d后,均产生明显的抑菌带,抑菌带宽度分别为7和10mm,培养10d后,仍然保持稳定的抑菌效果;抗真菌谱的结果表明,BHG、BTZ除对粉红单端孢无抑制作用外,对其他供试的病原真菌都有一定的拮抗作用,对8种供试病原真菌的菌落生长抑制率为8.12%～65.12%;通过生理生化特性研究表明,BHG、BTZ2种拮抗菌均可以产生淀粉酶、纤维素酶和脂肪酶3种胞外酶.The antagonistic bacterial strains BHG and BTZ was isolated from the microflora of grape cluster.The bacteriostatic effects against Botrytis cinerea were tested.The results showed that after 5 days of culture,two bacteriostatic belts of 7 mm and 10 mm in width were observed on the culture plate and the inhibitory activity maintained for 10 d.Antimicrobial spectrum indicated that the strains BHG and BTZ had a broad antifungal spectrum except Valsa japorica（Trichothecium roseum）,which could inhibit hyphal growth of at least 8 pathogens with inhibitive rate ranging from 8.12% to 65.12%.Properties of physiological and biochemical indicated that both BHG and BTZ produced amylase,cellulose and lipase.

关 键 词：葡萄灰霉病菌 对峙培养 胞外酶 拮抗细菌 

分 类 号：S182[农业科学—农业基础科学]