水杉木材DNA提取及条形码分子鉴定  

DNA extraction from wood Metasequoia glyptostroboides and molecule barcode identification

作　　者：杨星宇[1] 杨路路[1] 余志伟[1] 杨建明[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2011年第4期397-403,共7页Journal of Hubei University：Natural Science

基　　金：湖北大学教学研究项目(2010.9)资助

摘　　要：通过对水杉不同年限及不同部位的木材DNA提取及片段扩增实验,结果显示改良后的CTAB(十六烷基三甲基溴化铵)法、SDS(sodium dodecyl sulfate)法及高盐低pH法均可以用于水杉木材DNA的提取,经过纯化后的木材DNA可以进行片段扩增.在提取木材DNA过程中,边材比心材更适合,所提取的DNA数量和质量更有保障;试验显示水杉木材DNA分子大多为23kbp.运用DNA条形码筛选分析、序列特征分析、遗传距离秩和检验、barcoding gap检验,进行木材DNA分子系列物种鉴定,结果表明序列ITS2、trnL-F比较适合其DNA条形码技术要求,可以作为水杉木材鉴定序列.Through wood DNA fragments extraction and PCR experiment,in different fixed number of year and in different parts of Metasequoia glyptostroboides,the results showed the method of CTAB（16 alkyl three methyl brominated ammonium）,SDS（sodium dodecyl sulfate） and high-salt-low pH after improvement could be used in the Metasequoia glyptostroboides wood′s DNA extracted.After purification in wood DNA,the experiment proved it could be PCR.During the extracting,the sapwood was better than heartwood,sapwood DNA′s quantity and quality was more reliable.The experiments showed that the Metasequoia glyptostroboides wood′s DNA molecules was about 23 kbp.In the use of wood DNA molecular series,we carried the analysis of testing DNA sequence,bar code screening,characteristics of the genetic distance,rank and inspection,barcoding gap inspection,the results indicated that the sequence ITS2,trnL-F was suitable to its DNA bar code technology requirements.It could be used as identification Metasequoia glyptostroboides wood.

关 键 词：水杉 木材DNA提取 PCR DNA条形码 十六烷基三甲基溴化铵 

分 类 号：Q94-336[生物学—植物学]