1,25-二羟维生素D_3对哮喘大鼠BALF中Eotaxin及IL-8表达的影响  

Effects of 1,25-(OH)_2D_3 on eotaxin and IL-8 in BALF of asthmatic rat

作　　者：田维敏[1] 尚云晓[2] 蔡栩栩[2] 魏秀清 

机构地区：[1]厦门大学附属中山医院儿科,厦门361004 [2]中国医科大学附属盛京医院小儿呼吸内科,沈阳110004 [3]牡丹江市妇女儿童医院儿科,牡丹江157000

出　　处：《实用药物与临床》2011年第6期451-454,共4页Practical Pharmacy and Clinical Remedies

基　　金：沈阳市科学技术计划项目(1081269-9-00)

摘　　要：目的观察1,25-(OH)2D3对哮喘大鼠BALF中Eotaxin及IL-8表达的影响。方法 50只Wistar大鼠随机分为正常对照组(N)、哮喘组(A)、1,25-(OH)2 D3组(VD)、布地奈德组(B)及1,25-(OH)2 D3+布地奈德联合治疗组(L),每组10只。用ELISA法检测BALF中Eotaxin及IL-8的表达水平。结果各组Eotaxin和IL-8表达比较:A组较其他各组明显增高(P<0.01、P<0.05);VD组较A组明显降低(P<0.05),但仍高于N组、B组及L组(P<0.01);L组较A组、VD组及B组明显减低(P<0.05、P<0.01)。结论哮喘急性发作时,BALF中Eotaxin及IL-8的表达水平显著增高,1,25-(OH)2D3有效抑制了哮喘时的Eotaxin和IL-8的表达水平,与布地奈德联合治疗作用更为明显。Objective To observe the effects of 1,25-（OH）2D3 on the levels of eotaxin and IL-8 in BALF of asthmatic rats.Methods 50 Wistar rats were randomly divided into five groups：Normal control group（N）,asthma group（A）,1,25-（OH）2D3 group（VD）,budesonide group（B）,and therapeutic alliance group of 1,25-（OH）2D3 and budesonide（L）,10 rats for each group.The expressive levels of eotaxin and IL-8 in BALF were detected by ELISA.Results The levels of eotaxin and IL-8 increased more significantly in group A than those of other groups（P0.01,P0.05）;the levels in group VD decreased markedly compared with group A（P0.05）,but remarkably higher than group N,group B and group L（P0.01）;the levels of group L decreased more markedly than those of group A,group B and group VD（P0.05,P0.01）.Conclusion The levels of eotaxin and IL-8 in BALF of acute episodes of asthma significantly increased.1,25-（OH）2D3 can efficiently inhibit the levels of eotaxin and IL-8 in asthma,and the combination of 1,25-（OH）2D3 and budesonide has a more significant effect.

关 键 词：哮喘 1 25-二羟维生素D3 嗜酸性粒细胞趋化蛋白 白介素-8 

分 类 号：R562.25[医药卫生—呼吸系统]