RP-HPLC法测定参芪肝康胶囊中水飞蓟宾的含量  

Simultaneous determination of silybin in shenqigankang capsules by RP-HPLC

作　　者：王婷[1] 于雪[2] 李飞[1] 

机构地区：[1]沈阳药科大学高等职业技术学院,沈阳110016 [2]中国医科大学附属盛京医院药学部,沈阳110004

出　　处：《实用药物与临床》2011年第6期492-493,共2页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立反相高效液相色谱法测定参芪肝康胶囊中水飞蓟宾的含量。方法色谱柱:Dia-monsil C18(200 mm×4.6 mm,5μm);流动相:甲醇-0.5%醋酸(48∶52,v/v);流速:1.0 mL/min;柱温:25℃;检测波长287 nm。结果水飞蓟宾的线性范围:12.0～240μg/mL(r=0.999 7),平均回收率为97.0%(RSD=1.9%)。结论该方法专属性强,结果准确,可用于参芪肝康胶囊的质量控制。Objective To establish a RP-HPLC method for the determination of silybin in Shenqigankang capsules.Methods A Diamonsil C18 column（200 mm×4.6 mm,5 μm）was used.The mobile phase：methanol-0.5% glacial acetic acid solution（48∶ 52,v/v）,and the flow rate was 1.0 mL/min.Detection was performed at wavelength of 287 nm under 25 ℃.Results The calibration curves were linear in the ranges of 12.0~240 μg/mL（r=0.999 7）.The average recovery rate was 97.0%（RSD=1.9%）.Conclusion This method is accurate and the exclusive for quality control of Shenqigankang capsules.

关 键 词：RP-HPLC 参芪肝康胶囊 水飞蓟宾 

分 类 号：R286.0[医药卫生—中药学]