产超广谱酶大肠埃希菌和肺炎克雷伯菌对头孢替坦等抗菌药物的耐药性研究  

Study on antimicrobial resistence of cefotetan against Extended-spectrum β-lactamase of Escherichia coil and Klebsiella pneumonia

作　　者：俞汝佳[1] 吕晓菊[1] 高燕渝[1] 宗志勇[1] 王晓辉[1] 

机构地区：[1]四川大学华西医院感染性疾病中心人类疾病生物治疗国家重点实验室感染性疾病研究室,四川成都610041

出　　处：《四川医学》2011年第12期1862-1864,共3页Sichuan Medical Journal

摘　　要：目的初步探讨头孢替坦等8种临床常用抗菌药物对产超广谱酶(ESBLs)的大肠埃希菌及肺炎克雷伯菌的体外抗菌活性,为临床合理用药提供依据。方法四川大学华西医院抗感染药物研究室分离自临床感染患者的120株经双纸片法确证为产ESBLs大肠埃希菌及肺炎克雷伯菌,采用琼脂二倍稀释法进行8种临床常用抗菌药物体外抗菌活性试验。结果亚胺培南等碳青霉烯类抗生素对产ESBLs抗菌作用有较强的抗菌活性,大肠埃希菌的MIC50为<0.03μg/ml,MIC90为0.06μg/ml,肺炎克雷伯菌的MIC50为<0.03μg/ml,MIC90为0.06μg/ml;头孢替坦对大肠埃希菌及肺炎克雷伯菌也有很好的抗菌活性,MIC50分别为0.06和0.06μg/ml,MIC90分别为1和2μg/ml。结论头孢替坦对临床常见产ESBLs的大肠埃希菌和肺炎克雷伯菌具有良好的抗菌活性。Objective To determine the vitro activity of the 8 antibiotics including eefotetan against clinical isolates of extended spectrum beta-laetamases（ESBLs） of Escherichia colt and klebsiella peneumoniae and provide experimental evidences for rational use of antibiotics. Methods A total of 120 strains of acinetobacter baumannii were isolated from infect room of west china hospital . The MIC of 8 antibiotics were evaluated by double-agar dilution method. Results Among the 120 isolatess, the re sistance rates to impenen is better than other antibiotics, MICso of Escherichia colt was 〈 0.03μg/ml, MIC90 was 0.06μg/ml; MIC50 of Klehsiella Peneumoniae was 〈0. 03μg/ml, MIC50 was 0. 06μg/ml, cefotetan was similar to impenen, MIC50 of Escherichia colt and klebsiella peneumoniae were 0.06μg/ml and 0.06μg/ml, MIC 90 were 1 μg/ml and 4μg/ml. Conclusion Cefotetan is a good antibiotic activity for the treatment to ESBLs of Escherichia colt and klebsiella peneumoniae fiffections.

关 键 词：ESBLS 大肠埃希菌 肺炎克雷伯菌 头孢替坦 体外抗菌活性 

分 类 号：R446.5[医药卫生—诊断学]