光谱法研究阿莫西林及其与DNA的相互作用  

Study on Amoxicillin and the Interaction with DNA by Spectrometric Methods

作　　者：康倩倩[1] 周光明[1] 

机构地区：[1]西南大学化学化工学院发光与实时分析教育部重点实验室,重庆400715

出　　处：《光散射学报》2011年第4期368-375,共8页The Journal of Light Scattering

基　　金：国家科技重大专项(2008ZX07315);重庆市自然科学基金(CSTC2007BB5370)

摘　　要：报道了阿莫西林对照品与阿莫西林胶囊的常规拉曼光谱(NRS)和在银胶基底上溶液的表面增强拉曼光谱(SERS),归属了各个振动峰位和增强峰位;研究了阿莫西林对照品溶液与DNA相互作用的荧光光谱和表面增强拉曼光谱。结果表明:阿莫西林胶囊与阿莫西林对照品的NRS及SERS图谱基本一致,说明胶囊中的辅料对阿莫西林的检测几乎没有影响;在SERS中,阿莫西林分子是通过氮原子和苯环平行吸附在银纳米表面;阿莫西林分子与DNA相互作用的主要键合模式是插入作用,阿莫西林中的苯环和氮原子插入到DNA双螺旋结构的碱基对之间,为深入了解此类药物的作用机理提供了十分重要的信息和有益的参考。Normal Raman spectroscopy（NRS） and surface-enhanced Raman spectroscopy （SERS） of amoxicillin and amoxicillin capsule on the silver colloidal substrate were repor- ted, and the vibrational and enhanced peaks were assigned~ the interaction of amoxicillin and DNA were investigated by Fluorescence and SERS. The results showed that, NRS and SERS of amoxicillin and amoxicillin capsule were consistent basically, which indicated that excipient hardly affected the detection of amoxicillin~ in SERS, amoxicillin were ab- sorbed on silver colloid through the nitrogen atom and the benzene ring parallel~ The in- teraction was mainly caused by the inserting-action mode between amoxicillin and DNA, and the benzene ring and the nitrogen atom of amoxicillin insert the base pair of the double helix structure of DNA, which provided important information and useful reference for un- derstanding deeply the mechanism of action of this kind of drug.

关 键 词：拉曼光谱 表面增强拉曼光谱 荧光光谱 阿莫西林 DNA 

分 类 号：O657.3[理学—分析化学]