脂肪酶在复合模板剂下氨基改性介孔SiO_2中的固定化活性  

Immobilization of Lipase on Amino-functionalized Mesoporous Silica Prepared Using a Composite Template

作　　者：王春凤[1] 周国伟[1] 李天铎[1] 许云强[1] 宋宏斌[1] 张磊[1] 

机构地区：[1]山东轻工业学院山东省轻工助剂重点实验室材料科学与工程学院,山东济南250353

出　　处：《青岛科技大学学报（自然科学版）》2011年第6期605-609,613,共6页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(20976100);山东省自然科学基金项目(ZR2010BM013);山东省科技发展计划项目(2010GGX10306)

摘　　要：以三嵌段聚合物(P123)和表面活性剂聚乙二醇(PEG 4000)为复合模板剂,使用水热法合成了高度有序的介孔SiO2(MS),并用3-氨丙基三乙氧基硅烷(APTES)对其进行后接枝改性。通过XRD、SEM、TEM和氮气吸脱附等手段对介孔SiO2进行了表征。结果表明,该介孔材料具有高度有序的六方状介孔结构。当介孔材料经表面氨基改性后,其孔径、孔体积和比表面积均相应降低,但其形貌和介孔结构仍保持不变。以该材料为载体对假丝酵母脂肪酶(CRL)固定化,考察了改性前后酶固定量和固定化酶的催化活性。结果显示改性后介孔SiO2固定化酶具有较好的催化活性和重复利用性,重复利用5次后,该固定化酶仍具有其最初活性的50%以上。Mesoporous silica（MS） was synthesized via hydrothermal process using triblock copolymer Pluronic P123 and poly（ethylene glycol）（PEG 4000） as dual templates.The materials were functionalized with（3-aminopropyl）triethoxysilane APTES by post-synthesis-grafting method.The results indicated that mesoporous silica possessed a 2-dimensional hexagonal mesoscopic structure.The mesoscopic structure could be remained,while pore diameters,pore volumes,and surface areas were decreasing after the functionalization.Candida rugosa lipase（CRL） was used as a model enzyme for studying the effect of amino-functionalized in loading amount and enzymatic activity.As a result,APTES functionalized mesoporous silica immobilized CRL showed higher hydrothermal stability and the greatest reusability,and its residual activity was maintained at levels of 50% to the initial value after the fifth reuses.

关 键 词：介孔二氧化硅 氨基改性 假丝酵母脂肪酶 固定化 

分 类 号：Q814.2[生物学—生物工程]