石斑鱼神经坏死病毒传播途径阻断的初步研究  

Preliminary study on blocking transmission of NNV in Epinephelus coioides

作　　者：林克冰[1] 方琼珊[1] 吴建绍[1] 周宸[1] 何丽斌[1] 林琪[1] 王涵生[1] 

机构地区：[1]福建省水产研究所,福建厦门361013

出　　处：《福建水产》2011年第5期15-19,共5页Journal of Fujian Fisheries

基　　金：福建省海洋与渔业厅重点项目(闽海渔科0413号)

摘　　要：本文报告了斜带石斑鱼(Epinephelus coioides)苗种培育中亲鱼、卵、饵料等几个关键环节的病毒携带检测以及几种药物对病毒的消除效果的试验。用RT-PCR法检测斜带石斑鱼亲鱼的粪便、卵、仔鱼、稚鱼,饵料轮虫、桡足类中都有检出神经坏死病毒(nervous necrosis virus,NNV)。聚维酮碘5～10mg/L浸泡卵20min、浸泡轮虫和桡足类30min,盐酸吗啉胍3～5mg/L浸泡卵30min、浸泡轮虫和桡足类40min,聚维酮碘5mg/L+盐酸吗啉胍3mg/L浸泡卵20min、浸泡轮虫和桡足类30min可有效灭活所携带的神经坏死病毒活性。To study the effect of some medication on the elimination of nervous necrosis virus （ NNV）, this report detected NNV from the key parts of hatching Epinephelus coioides such as parent fish, eggs and feeds by the method of reverse transcription polymerase chain reaction （ RT - PCR）. The detection of the soil, eggs, larvae, juveniles, rotifers and copepods by RT - PCR indicated that the NNV were detected in all samples. NNV activity could be eradicated by some methods such as eggs, rotifers and copepods were completely immersed in a solution of 5 - 10 mg/L Povidone Iodine within 20min and 30min, respectively ; eggs, rotifers and copepods were completely immersed in a solution of 3 - 5 mg/L Moroxydine Hydrochloride within 30min and 40min; eggs, rotifers and copepods were completely immersed in a solution of 5mg/L Povidone Iodine and 3mg/L Moroxydine Hydrochloride within 20min and 30min.

关 键 词：斜带石斑鱼 神经坏死病毒 传播途径 阻断 

分 类 号：S965.334[农业科学—水产养殖]