中成药可溶性汞形态分析方法及样品结果测定  

Speciation methods and sample analysis of soluble mercury in traditional Chinese medicines

作　　者：徐陆正[1] 解清[1,2] 闫赖赖[1,2] 陈玉红[3] 王京宇[1,2] 

机构地区：[1]北京大学公共卫生学院,北京100191 [2]北京大学医药卫生分析中心,北京100191 [3]安捷伦科技(中国)有限公司,北京100102

出　　处：《实验技术与管理》2011年第12期40-43,共4页Experimental Technology and Management

基　　金："重大新药创制"科技重大专项课题"中药中农药残留;重金属检测与分析关键技术研究"(2009ZX09502-027)

摘　　要：建立了微波辅助提取、HPLC-ICP-MS联用分析中成药中二价汞和甲基汞的方法。分别比较了提取液中盐酸和L-半胱氨酸含量、提取温度和提取时间对提取结果的影响,得到了微波辅助提取的最优条件:体积分数为1%的盐酸10mL和质量分数为5%的L-半胱氨酸1mL作为提取液,控温70℃,微波辅助提取1h。该方法测得的二价汞和甲基汞的精密度分别为1.7%和4.4%,加标回收率分别为80.7%～99.5%和93.7%～95.8%。并应用该方法分析测定了20种、共49批次的含朱砂的中成药中可溶性汞的形态。A method with microwave-assisted extraction and HPLC-ICP-MS to analyse the inorganic mercury and methyl mercury in the traditional Chinese medicines（TCMs） has been built.The hydrochloric acid and L-cysteine concentration,the extraction temperature and extraction time have been investigated.The optimal conditions are as follows： 1% hydrochloric acid 10 ml and 5% L-cysteine 1 ml as extract,temperature control 70 ℃,and time control 1h.Using this method,the inorganic mercury and methyl mercury＇s RSDs（relative standard deviation） are 1.7% and 4.4%,and the standardized recoveries are 80.7~99.5% and 93.7~95.8%.Then,49 batches of 20 kinds TCMs which contain cinnabar have been determinated using this method.

关 键 词：中成药 可溶性汞 形态分析 

分 类 号：R917[医药卫生—药物分析学]