球毛壳菌素F对Poly(I∶C)诱导的RAW264.7细胞TNF-α水平的影响  

The Effect of Chaetoglobosin F on TNF-α Level by RAW264.7 Cells Induced by Poly(I∶C)

作　　者：赵俊丽[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,江苏南京211171

出　　处：《南京晓庄学院学报》2011年第6期69-72,共4页Journal of Nanjing Xiaozhuang University

基　　金：南京晓庄学院青年专项(2009NXY31)

摘　　要：该实验主要研究球毛壳菌素F对聚肌苷酸胞苷酸Poly(I∶C)诱导的小鼠巨噬细胞系RAW264.7 TNF-α表达水平的影响.采用不同浓度的Poly(I∶C)(50μg.mL-1、100μg.mL-1和200μg.mL-1)和球毛壳菌素F(2μg.mL-1)对RAW264.7进行处理,运用流式细胞术检测细胞内TNF-α的表达水平.结果表明,各浓度的Poly(I∶C)与对照组相比均能极显著提高RAW264.7的TNF-α水平(P<0.001),并且具有浓度依赖性;球毛壳菌素F能够极显著地降低Poly(I∶C)诱导的细胞TNF-α水平(P<0.01).结论,球毛壳菌素F可能是通过降低炎症因子TNF-α的分泌而达到抗炎作用的.The present study is to investigate the influence of chaetoglobosin F on TNF-α level induced by Poly（ I： C）. RAW264.7 cells were treated with various concentrations of Poly （ I： C ） （50μg/ml, 100μg/ml and 200μg/ ml） and chaetoglobosin F and then TNF-α expression levels of RAW264.7 were analyzed by flow eytometry. The result showed that all doses of Poly（ I： C） enhanced TNF-α production significantly compared with the control group with a dose dependent manner（ P 〈 0. 001 ）. Chaetoglobosin F decreased significantly the TNF-α expression of RAW264.7 cells induced by Poly（ I： C） （P 〈0.01 ）. It is concluded that ehaetoglobosin F plays an anti-inflammatory role partly through decreasing the secretion of TNF-α .

关 键 词：球毛壳菌素F 聚肌苷酸胞苷酸 肿瘤坏死因子Α 小鼠巨噬细胞系RAW264.7 

分 类 号：Q24[生物学—细胞生物学]