高效液相色谱法计量奶山羊粪中的植物激素  

Determination of Plant Hormones in Milchgoat Dungs by High Performance Liquid Chromatography

作　　者：陈立德[1] 蒋盛岩[1] 

机构地区：[1]邵阳学院生物与化学工程系,湖南邵阳422000

出　　处：《邵阳学院学报（自然科学版）》2011年第4期52-55,共4页Journal of Shaoyang University：Natural Science Edition

摘　　要：建立了高效液相色谱法计量奶山羊粪便中赤霉酸(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(Z)4种植物激素的方法.运用Hypersil-ODS C18(4.6 mm×250 mm,5μm)色谱柱,计量GA3I,AA,ABA的流动相为乙腈+磷酸;计量Z的流动相为乙腈+三乙胺;柱温35℃,GA3和IAA计量波长为208 nm,ABA为262 nm,Z为270 nm,流速1ml/min.计量结果表明,在此实验条件下能使奶山羊粪中GA3I,AA,ABA和Z 4种植物激素得到较好的分离,含量分别为6.483624μg/g,1.404628μg/g,0.1692108μg/g,0.340468 ng/g.A high performance liquid chromatographic method was used to metering 4 kinds of plant hormones of gibberellic acid （GA3）, indole-3-acetic acid （IAA）, abscisic acid （ABA） and zeatin （Z） in Shannan white Goat. A Hypersil,ODS C18（4.6 mm× 250 mm, 5 μ m） column was used with solution of ethylnitrile-phosphoric acid and ethylnitrile-triethylamine as mobil phase for detection GA3, IAA,ABA and Z; Column temperature was 35℃. Metering wavelength was 208 nm for GA3 and IAA, 262 nm for ABA, 270 nm for Z. The velocity of flow was lml/min. The results showed that 4 kinds of plant hormones were well separated. The contents of GA3, IAA, ABA and Z were respectively 为 6.483624 μ g/g, 1.404628 μ g/g,0.1692108 μ g/g,0.340468 ng/g.

关 键 词：高效液相色谱 奶山羊粪 植物激素 

分 类 号：Q945.31[生物学—植物学]