七氟烷对幼鼠认知功能及海马CA1区NMDAR1表达的影响  

Effects of sevoflurane on cognition function and expression of NMDAR1 in neonatal rats

作　　者：张永杰[1] 唐冬梅[1] 徐桂萍[2] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]新疆维吾尔自治区人民医院

出　　处：《山东医药》2011年第47期24-26,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81160016);新疆维吾尔自治区自然科学基金资助项目(2011211A056)

摘　　要：目的观察七氟烷麻醉对幼鼠认知功能的影响。方法将48只新生SD大鼠随机分为三组各16只,高浓度组吸入3%七氟烷6 h,低浓度组吸入1.5%七氟烷6 h,对照组吸入空气。各组分别于出生后21 d(Ⅰ期)、60 d(Ⅱ期)随机取8只行Y型迷宫试验,连续3 d记录全天总反应时间(TRT)和错误次数(EN);于最后一次测试后处死,采用免疫组化染色法测定脑海马CA1区N-甲基-D-天冬氨酸1(NMDAR1)表达情况。结果与对照组比较,高浓度组试验Ⅰ期T1时TRT延长,EN增加(P<0.05);Ⅱ期各组测试成绩差异无统计学意义;各组海马CA1区NMDAR1表达Ⅰ、Ⅱ期差异均无统计学意义。结论七氟烷麻醉后早期可导致幼鼠一过性认知功能减退,但对NMDAR1表达无明显影响。Objective To investigate the effects of inhalation of sevoflurane on cognitive function in neonatal rats.Methods Forty-eight new born SD rats were randomly divided into three groups with 16 rats each,the high concentration（H） group inhaled 3% sevoflurane for 6 h,the low concentration（L）group inhaled 1.5% sevoflurane for 6 h,the control group inhaled air.Cognitive function was assessed using Y-maze test at 21 d（Ⅰstage） and 60 d（Ⅱstage） in each group were randomly taken eight rats after anesthesia,on three consecutive days recording total reaction time and error number.The animals were killed immediately after the last Y-maze test and hippocampus was isolated,and the N-methyl-D-aspartate 1（NMDAR1） expression was determined using immuno-histochemical technique.Results Total reaction time（TRT） in group H（Ⅰstage） Y-maze was longer than that in control group,and error number（EN） increased（P〈0.05）;While Y-maze（Ⅱstage） test scores in each group showed no significant difference;Hippocampal CA1 NMDAR1 expression atⅠand Ⅱstage showed no significant difference.Conclusion Inhalation of sevoflurane can induce transient cognitive decline in early neonatal rats,but shows no significant effect to the expression of NMDAR1.

关 键 词：七氟烷 大脑海马 认知功能 N-甲基-D-天冬氨酸受体1 

分 类 号：R614.21[医药卫生—麻醉学] R338.64[医药卫生—外科学]