毛细管电泳电容耦合非接触电导检测器  

Design of Capillary Electrophoresis Capacitively Coupled Contactless Conductivity Detector

作　　者：曾凡亚[1] 苏建坡[1] 赵书俊[1] 张书胜[2] 张斌[1] 卢艳艳[2] 

机构地区：[1]郑州大学物理工程学院,河南郑州450001 [2]郑州大学化学系,河南郑州450001

出　　处：《仪表技术与传感器》2011年第12期3-5,23,共4页Instrument Technique and Sensor

基　　金：国家自然科学基金(21077095);电分析化学国家重点实验室开放基金(2009002)

摘　　要：设计了一种集激励、检测和数据采集功能于一体的毛细管电泳非接触电导检测器,采用文氏电桥振荡器产生激励信号,运用绝对值检波技术对检测信号进行处理,并设计USB数据采集电路和上位机软件,实现波形数据的采集、存储和图谱的绘制。介绍了其检测原理、电路结构及实现,使用葡萄糖(0.04 mol/L)+果糖(0.04 mol/L)+蔗糖(0.04 mol/L)样品进行了检测器重复性实验,并分离检测了6种酚酸,对检测器检测限作出评定。实验结果表明:峰保留时间和峰面积相对标准差分别小于1%和7.5%,对酚酸的最低检测限为5μg/mL(信噪比S/N=3),检测器灵敏度高、重复性好。A kind of capillary electrophoresis capacitively coupled contactless conductivity detector（CE-C4D） was designed,which had signal source,detection circuit and data acquisition unit.Wien bridge oscillator was used to get a signal source,and absolute value demodulation was used to process signals.And a data acquisition circuit with USB interface and software were designed to acquire and store the signal data and draw spectrum.The principle and circuit of CE-C4D were also introduced,glucose（0.04 mol/L）＋fructose（0.04 mol/L）＋sucrose（0.04 mol/L） were took as samples to test the repeatability and at the same time,six kinds of phenolic acids were separated and determined to evaluate the detection limit.The results show that the relative standard deviation（RSD） of peak retention time and area are less than 1% and 7.5%,and the detection limit of six kinds of phenolic acids is 5 μg/mL（signal noise ratio,S/N=3）,the detector has good repeatability and high sensitivity.

关 键 词：毛细管电泳 非接触电导检测 文氏电桥振荡器 绝对值检波 

分 类 号：TH832[机械工程—仪器科学与技术]