宁夏枣树花药离体培养再生体系的建立  

The Anther of Ningxia Chinese Jujube Culturing in vitro and Establishment of Regeneration System

作　　者：王敬东[1,2] 陈晓军[1,2] 张丽[1,2] 石磊[1] 马洪爱[1] 李苗[1] 甘晓燕[1] 宋玉霞[1,2] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《中国农学通报》2011年第31期174-178,共5页Chinese Agricultural Science Bulletin

基　　金：北京林业大学林木育种国家工程实验室开放课题"宁夏酿酒葡萄花药脱毒及病毒分子检测关键技术研究"(FOD2010-b);宁夏回族自治区科技攻关资助项目"农作物与经济植物分子育种研究"(KGZ-16-07-02)

摘　　要：采用宁夏3个特色枣树品种的花药为外植体,探索MS培养基中不同激素、激素的不同浓度配比对愈伤组织诱导、不定芽分化的影响,建立其愈伤组织诱导及不定芽再生体系。结果表明,细胞分裂素6-BA对宁夏枣树胚性愈伤组织诱导起重要作用,AgNO3对愈伤组织分化不定芽影响显著。经过正交试验分析得出,最佳诱导培养基为MS+6-BA1.0 mg/L+2,4-D1.0 mg/L+IBA0.4 mg/L;最佳分化培养基是:MS+6-BA1.5 mg/L+IBA0.6 mg/L+AgNO30.5 mg/L。在最佳诱导培养基中灵武长枣诱导率最高,达89.0%,在最佳分化培养基中同心圆枣分化率最高,达88.0%。Experiment was conducted with anthers of three lines of Ningxia Chinese jujube,culturing in vitro and gained some regeneration plant,for studying the effects of different phytohormone and their levels in MS medium on the callus induction and adventitious bud regeneration.The results showed that 6-BA acted as one of important factor in induce embryogenic callus of Ningxia Chinese jujube.After orthogonal test analysis,the best induce medium was MS＋6-BA 1.0 mg/L＋2,4-D 1.0 mg/L＋IBA 0.4 mg/L.AgNO3 played a important role in differentiation.The suitable medium was MS＋6-BA 1.5 mg/L＋IBA 0.6 mg/L＋AgNO 3 0.5 mg/L.Lingwu Long jujube had the highest inductivity in the best induced medium which was 89%;Tongxin round jujube had the highest differentiation rate in the best differential medium which was 88%.

关 键 词：枣树 花药 离体培养 再生体系 

分 类 号：S662.9[农业科学—果树学]