兔气管移植组织一氧化氮浓度测定及意义  

The significance and determination of nitric oride concentration in rabbit trached transplant tissue

作　　者：卢景彤[1] 程飞[1] 徐世东[1] 

机构地区：[1]厦门市第三医院胸外科,厦门361100

出　　处：《实用肿瘤学杂志》2011年第6期528-530,共3页Practical Oncology Journal

摘　　要：目的 探讨移植气管组织中一氧化氮浓度与管腔狭窄的相关性,以及应用免疫抑制剂对两因素的影响.方法 将48只雄性大白兔随机分为A、B两组,每组24只.A组常规行气管移植术;B组气管移植术后给予免疫抑制剂环磷酰胺.两组兔分别于术后第3天、第7天、第14天取8只兔子处死.分别测定术后不同时间两组移植气管标本中的NO浓度,并测定移植气管的通畅度.结果 B组兔移植气管狭窄较A组轻,术后第7天、第14天两组间差别显著（P＜0.01）.B组术后不同时间移植段气管NO水平与A组比较均明显降低（P＜0.05）.结论 移植气管组织中一氧化氮浓度与管腔狭窄的程度有相关性;免疫抑制剂能减少NO的产生,对减轻移植段气管术后狭窄有帮助.Objectives To approach the correlation between the NO concentration and stenosis of tracheal lumens in the tracheal graft tissue, and the effect on the two factors with immunosuppressant. Methods Forty eight male normal albino rabbit were divided into group A and B, randomly and equally. Tracheal segment were allotransplanted in two groups. No immunosuppressant was given in group A, The recipients were treated with cyclophosphamide in groap B. Eight rabbits of each group were sacrificed at the third day, seventh day and four- teenth day after operation, and the NO concentration and the percent patency in these postmortem specimens were examined totally. Results Tracheal stenosis was found more serious in group A than in group B, There was sig- nificance in the difference between the seventh and fourteenth day after operation （ P 〈 0.01 ）. At different time （the seventh and the fourteenth day）after operation ,the NO concentration in group B was significantly lower than in group A （ P 〈 0.05 ）. Conclusion The correlation was found between the NO concentration and stenosis of tracheal lumens in the tracheal graft tissue;Immunosuppressive drugs can reduce the production of NO, and help to relieve the tracheal stenosis after operation.

关 键 词：移植气管 一氧化氮 免疫抑制剂 兔 

分 类 号：R655.3[医药卫生—外科学]