承德地区两温泉中古细菌基因型多样性分析  

Archaeal Diversity Analysis for the Two Hot Springs in Chengde Area of China

作　　者：郝春博[1] 张丽娜[1] 李思远[1] 周训[1] 冯传平[1] 方斌[1] 

机构地区：[1]中国地质大学水资源与环境学院,北京100083

出　　处：《现代地质》2011年第6期1207-1213,共7页Geoscience

基　　金：国家自然科学基金项目(40802059;40972163);中央高校基本科研业务费专项资金项目(2010ZD03;2011YXL035);教育部高等学校博士学科点-新教师基金项目(200804911008)

摘　　要：通过构建16S rDNA克隆文库,对承德地区两个不同温度的温泉中古细菌的基因型多样性进行了分子生态学研究。结果表明:山湾子温泉(A12)74.5℃热水中的古细菌主要分属泉古菌门(Crenarchaeota)和广古菌门(Euryarchaeota)两个门;七家温泉(A14)61.4℃热水中的古细菌则只属于泉古菌门,没有广古菌门微生物的分布。样品A12中的古细菌序列只可分为3种基因型,而A14中的古细菌序列可分为10种基因型。古细菌多样性的差异表明,温度是影响温泉中古细菌多样性水平的重要因素。样品A12中古细菌群落具有厌氧发酵乙酸产甲烷的生理功能,而A14中大多数古细菌均与氨氧化古菌有密切的亲缘关系,其主要生理功能为好氧氨氧化。The archaeal diversity of two hot springs with different temperature in Chengde area of China was investigated using 16S rDNA clone library.The results showed that the archaea in the Shanwanzi hot spring A12（74.5 ℃） belonged to two phyla： Crenarchaeota and Euryarchaeota,but the archaea in the Qijia hot spring A14（61.4 ℃） belonged to Crenarchaeota only.Based on sequence similarity,a total of 3 phylotypes or operational taxonomic units（OTUs） were obtained from A12 sample,while the archaeal sequences in A14 sample could be classified into 10 phylotypes.The biodiversity difference between the two samples revealed that the temperature might be an important factor to affect the level of archaeal diversity in hot springs.The major physiological function of archaea in sample A12 was methanogenesis through anaerobic acetate fermentation.On the contrary,the most archaea in sample A14 were closely related to ammonia-oxidizing archaea,indicating that their dominant physiological function was aerobic ammonia oxidation.

关 键 词：温泉 16S RDNA 克隆文库 古细菌 生物多样性 承德地区 

分 类 号：P641[天文地球—地质矿产勘探] Q939.1[天文地球—地质学]