四君子汤含药血清与氟尿嘧啶合用对胃癌细胞SGC-7901的生长抑制作用  

Experimental Study of Sijunzi Decoction Medicated Serum and Fluorouracil on Growth Inhibiting of SGC-7901 cells

作　　者：黄浏姣[1] 洪亚群[1] 赵刚[1] 

机构地区：[1]湖北中医药大学,湖北武汉430061

出　　处：《湖北中医药大学学报》2011年第6期3-5,共3页Journal of Hubei University of Chinese Medicine

基　　金：湖北省自然科学基金项目(2005ABA190)

摘　　要：目的观察四君子汤含药血清和氟尿嘧啶(FU)合用对体外培养的人胃癌细胞SGC-7901生长与增殖的抑制效应。方法将低、中、高3种剂量的四君子汤含药血清与FU配伍分别作用于3个实验组的人胃癌SGC-7901细胞,另设不含药血清组和FU单用组,共计5个组,药物作用时间均为24h。采用倒置显微镜观察各组SGC-7901细胞的生长状态与形态改变;用MTT方法计算各组细胞的生长抑制率。结果药物处理组细胞变小、变圆、悬浮等情况明显高于对照组;四君子汤含药血清与FU合用各组对SGC-7901细胞的生长抑制作用明显高于FU单用组,并呈剂量依赖性。结论四君子汤能增强FU对胃癌SGC-7901细胞生长的抑制效果。Objective To investigate the effect of Sijunzi decoction medicated serum combined with fluorouracil on the growth and pro- liferation of human carcinoma cell line SGC -7901 in vitro. Methods Five groups were set for the study as： FU combined high, medium, low dose groups, nonmedicared serum group, and the FU group. The human gastric carcinoma cell line SGC -7901 were treated with Sijunzi decoction medicated serum combined with fluorouracil for 24 hours respectively. The morphological changes of SGC -7901 ceils were observed with invert microscope,The effects of Sijunzi decoction medicated serum combined with fluorouracil on the growth of SGC -7901 cells were detected by MTT assay. Results Comparing with controls, the growth and proliferation of SGC - 7901 cells were significantly inhibited by Sijunzi decoction combined with fluorouracil, and the growth inhibiting rates to SGC -7901 cells were revealed in a medicated serum dose - dependent manner. Conclusion The combination of Sijunzi decoction and fluorouracil can enhanced the growth inhibitive effect of FU to human gastric carcinoma SGC -7901 cells.

关 键 词：四君子汤 氟尿嘧啶 SGC-7901细胞 生长抑制 

分 类 号：R285.5[医药卫生—中药学]