根结线虫侵染对Micro-Tom番茄同工酶变化的研究  

Changes of Isozyme in Meloidogyne incognita Infected Micro-Tom Tomato

作　　者：洪权春[1] 

机构地区：[1]商丘师范学院生命科学系,河南商丘476000

出　　处：《湖南农业科学》2011年第12期39-41,共3页Hunan Agricultural Sciences

基　　金：河南省科技厅国际合作项目(094300510061)

摘　　要：用根结线虫卵块接种盆栽Micro-Tom番茄,接种2 d后开始取样,共取样10次。通过显微观察和酶谱分析,研究了Micro-Tom番茄体内的SOD和POD同工酶谱带及活性变化与根结线虫侵染的关系。结果显示,感染南方根结线虫后,番茄植株内POD同工酶的谱带数量显著增多,且随接种后天数的增加,酶活性增强;而SOD同工酶谱带数量虽没有明显变化,但其活性也明显增强。这说明SOD和POD同工酶在植株受到病害感染时做出了适当反应,以抵御病害。Micro-Tom tomato seedlings were inoculated with Meloidogyne incognita eggs in laboratory. The first sampling was at the second day after inoculation, altogether 10 samples were taken. The technology of PAGE was used to analyze the changes of isozyme bands and enzyme activities of SOD and POD in the infected Micro-Tom tomato by Meloidogyne incognita. The results showed that after the Micro-Tom tomato infected by Meloidogyne incognita, the isozyme bands of POD in tomato plant significantly increased, and the activity of POD increased with days passing; the isozyme bands of SOD had no obvious change, but its activity also significantly increased. Therefore, the results indicated that the enzymes of SOD and POD in tomato infected by Meloidogyne incognita had made adaptive response in order to resist infection.

关 键 词：南方根结线虫 Micro—Tom番茄 超氧化物歧化酶 过氧化物酶 同工酶 

分 类 号：S641.2[农业科学—蔬菜学]