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作 者:程许娜[1] 王鹏霄[1] 张静[1] 苏金乐[1]
机构地区:[1]河南农业大学,河南郑州450002
出 处:《湖南农业科学》2011年第12期117-120,共4页Hunan Agricultural Sciences
基 金:河南省重点科技攻关计划项目(0624070035)
摘 要:采用PEG-6000模拟干旱胁迫,测定2年生樟树幼苗叶片的生理生化指标,研究樟树的抗旱机理。结果表明:随着胁迫程度的加强和时间的延长,叶片中丙二醛(MDA)的含量增幅先慢后快,SOD活性表现为"升-降-升-降";CAT活性呈现"升-降-升"的趋势;POD活性的变化呈现先升后降。这3种保护酶是相互协调而起作用有效的清除自由基,减轻干旱胁迫对细胞膜的伤害,增加了樟树的抗旱性。此外,可溶性蛋白也是处于先升后降的变化。试验证明樟树对干旱胁迫有一定的适应性。Simulating drought stress with PEG-6000, and the physiological and biochemical indexes of seedling leaf of two-year-old Cinnamomum camphora were determined to study drought resistance mechanism of Cinnamomum camphora. The results indicated that, with drought stress intensified and time passed, the content of MDA firstly increased slowly and then increased quickly; the activity of SOD showed a repeated rising and falling tendency; the activity of CAT showed a rising-falling-rising tendency; the activity of POD increased firstly and then decreased tendency. The concerted reaction of these three protective enzymes could eliminate free radicals effectively, which might alleviate the damage to epicyte under drought stress, and increase the drought resistance of Cinnamomum camphora; in addition, the change of soluble protein also increased firstly and then decreased. The above results indicated that Cinnamomum camphora could be adapted to the drought stress conditions.
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