不同因素对互叶白千层丛生芽诱导的影响  

Effects of Various Factors on Multiple Shoot Induction of Melaleuca leucadendron

作　　者：莫昭展[1] 杨福[1] 苏建睦[1] 王云波[1] 许凤[1] 

机构地区：[1]玉林师范学院生命科学与生物技术二级学院,广西玉林537000

出　　处：《河南农业科学》2011年第12期136-139,共4页Journal of Henan Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0992011-13);广西青年科学基金项目(桂科青0832099)

摘　　要：研究了不同基本培养基、碳源及其质量浓度、稀土元素、6-BA和2,4-D配比对互叶白千层丛生芽诱导的影响。结果表明,诱导效果较好的是MS培养基。以20g/L的葡萄糖代替其他浓度的糖类,能有效提高白千层丛生芽诱导率。稀土元素对丛生芽的诱导有一定的促进作用,以5mg/L的硝酸铈效果较好,诱导率为93.33%。在添加1.0mg/L 6-BA和0.01mg/L 2,4-D的MS培养基上丛生芽诱导率高达100%。In this paper,the influences of several factors,various basal media,carbon source,rare earth elements,6-BA and 2,4-D combinations,on the multiple shoot induction of Melaleuca leucadendron was studied.The results showed that MS culture medium gave the best result and was effective to improve the induction rate with the 20g/L glucose than other concentrations of carbon sources.The rare earth elements had promoted effect on the multiple shoot induction with the concentration of 5mg/L Ce（NO3）3 and the induction rate of 93.33%.The induction rate was up to 100% on MS culture media added with 1.0mg/L 6-BA and 0.01mg/L 2,4-D.

关 键 词：互叶白千层 丛生芽诱导 葡萄糖 稀土元素 

分 类 号：S794.9[农业科学—林木遗传育种]