响应曲面法优化微波辅助提取淫羊藿中淫羊藿苷工艺  

Optimization of Microwave-assisted Extraction Process for Icariin from Epimedium by Response Surface Methodology

作　　者：梁新丽[1] 王春柳[1] 王光发[1] 赵国巍[1] 廖正根[1] 杨明[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2012年第1期11-15,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：863计划中药生产创新工艺及装备研发项目(2009AA043202)

摘　　要：目的:探讨微波辅助提取技术在叶类中药材中的适宜性。方法:以淫羊藿为例,采用Box-Behnken设计考察微波功率、料液比和提取时间3个因素对淫羊藿苷提取率及干浸膏得率的影响,采用响应曲面法优化微波辅助提取淫羊藿苷得提取工艺,并与传统提取工艺比较。结果:淫羊藿苷提取率和干浸膏得率与所考察3因素之间关系符合二次模型。确定最佳提取工艺为微波功率800 W,料液比1∶16,提取时间37 min。该条件下淫羊藿苷提取率84.53%,干浸膏得率22.47%。结论:微波辅助提取淫羊藿苷具有时间短、提取率高、化学特征峰无差异、能量消耗更低等特点,微波辅助技术适用于淫羊藿等叶类药材的提取。Objective：To investigate suitability of microwave-assisted extraction technology used in leaf Chinese medicine.Method：Epimedium was taken as example.Three factors were investigated influence of microwave power,solvent-solid ratio,extraction time on yield of extracted for icariin and yield of dry extract by Box-Behnken design.Microwave-assisted Extraction Process for icariin was optimized by response surface design,and compared with traditional extraction technique.Result：Relation fitted quadratic model between yield of extraction for icariin,yield of dry extract and 3 factors investigated.Optimum extraction technology of icariin was：microwave power 800 W,solvent-solid ratio 16,extraction time 37 min.Under these conditions,average yield of icariin was 84.53%,dry extracta sicca was 22.47%.Conclusion：Microwave-assisted extraction of icariin had characteristics of low in time,high extraction ratio,no difference of chemical characteristics peaks and lower energy consumption.Microwave-assisted technology applied to extraction of leafy medicinal materials such as Icariin.

关 键 词：微波辅助提取 淫羊藿苷 响应曲面法 BOX-BEHNKEN设计 

分 类 号：R283.6[医药卫生—中药学]