缩泉丸对自然衰老大鼠逼尿肌β_3-肾上腺素能受体mRNA表达的影响  

Effects of Suoquan Wan on β_3-ARmRNA Expression of Detrusor in Natural Aging Rats

作　　者：操红缨[1] 吴君[2] 吴清和[1] 黄萍[1] 

机构地区：[1]广州中医药大学,广州510006 [2]山东中医药高等专科学校,山东烟台264100

出　　处：《中国实验方剂学杂志》2012年第1期125-128,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873425);广东省中医药局科研项目(2009230)

摘　　要：目的:观察自然衰老大鼠逼尿肌β3-肾上腺素能受体(AR)mRNA表达的改变,探讨缩泉丸"缩尿"的机制。方法:取老年大鼠(20月龄)50只,青年大鼠(3月龄)10只。设置组别为:空白对照组、模型对照组、金匮肾气丸组(1.08 g.kg-1.d-1)、缩泉丸高、中、低剂量组(0.293,0.585,1.170 g.kg-1.d-1),每组10只。空白对照组和模型对照组给予同体积蒸馏水,缩泉丸低、中、高剂量组给予相应浓度的缩泉丸药液,金匮肾气丸组予金匮肾气丸药液,连续4周。选用代谢笼法测量大鼠24h尿量及水负荷5 h尿量,采用PCR方法检测自然衰老大鼠逼尿肌β3-AR mRNA表达。结果:缩泉丸中、高剂量组动24 h尿量明显减少,分别为(4.18±2.07),(4.16±1.54)mL.(100g)-1;缩泉丸低、中、高剂量组水负荷后5 h内尿量明显减少,分别为(1.08±0.43),(0.99±0.54),(0.96±0.49)mL.(100g)-1;自然衰老大鼠逼尿肌β3-AR mRNA表达明显下降(P<0.05),相对比值为1.61±0.045;给予缩泉丸后,中、高剂量可以显著提高自然衰老大鼠膀胱逼尿肌中β3-AR mRNA的表达(P<0.05),相对比值为1.63±0.036,1.70±0.061。结论:缩泉丸可以上调自然衰老大鼠逼尿肌β3-ARmRNA的表达,可能是其发挥"缩尿"作用的机制。Objective：The mechanism of Suoquan Wan（SQW） reducing urination was investigated by observing the change of the expression of β3-adrenergic receptor（AR）of mRNA in bladder detrusor of the natural aging rat.Method：Fifty aged rats（20 months） and 10 young rats（3 months） were chosen.Young rats were chosen as control,aged rats were randomly divided into five groups：model,Jinkui Shenqi pill group,SQW high,middle and low dose group,10 rats in each group.The low,middle and high dose group of SQW were given the appropriate concentration（0.293,0.585,1.170 g·kg^-1·d^-1）.Jinkui Shenqi pill group was given appropriate liquid（1.08 g·kg^-1·d^-1）,once everyday for four weeks.The urination after the water load in rats at 5 h and 24 h was measured in metabolic cages.β3-AR mRNA expression was detected by PCR in the detrusor of the natural aging rats.Result：The 24 h urination of SQW middle and high dose group significantly decreased.The 5h urination after the water load in rats of SQW low,middle and high dose group also significantly decreased.β3-AR mRNA expression significantly decreased in the detrusor of the natural aging rats（P〈0.05）.SQW high dose can remarkably elevate β3-AR mRNA expression in the detrusor of the natural aging rats after administration SQW.Conclusion：SQW can remarkably elevate β3-AR mRNA expression in the detrusor of the natural aging rats,which was the possible mechanism of SQW reducing urination.

关 键 词：缩泉丸 自然衰老大鼠 β3-肾上腺素能受体mRNA 

分 类 号：R285.5[医药卫生—中药学]