粘细菌的分离鉴定及活性检测  

The Isolation,Identification and Activity Assays of the Myxobacteria

作　　者：常宁宁[1] 欧一新[1] 徐庆妍[1] 钱晓鸣[1] 郑忠辉[1] 黄耀坚[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2012年第1期112-116,共5页Journal of Xiamen University：Natural Science

基　　金：科技部国际合作项目(2007DFA30970);中央高校基本业务费(2010121092)

摘　　要：近年来,粘细菌由于其次级代谢产物结构新颖、生物活性多样而备受关注,成为发展新药的重要来源.利用已灭活酵母菌或大肠杆菌(Escherichia coli)诱导法和滤纸片诱导法等方法进行粘细菌的分离,并采取多种方法对所分离出菌株进行纯化,从95份土样中共分离出118株粘细菌,纯化出54株.根据16SrDNA进行菌株鉴定,主要的粘细菌为粘球菌属(Myxococcus)、珊瑚菌属(Corallococcus)、多囊菌属(Polyangium)和堆囊菌属(Sorangium).对获得纯化的菌株进行了抗菌抗肿瘤活性测定,结果显示有46.3%的菌株具有抗菌作用,有66.7%的菌株具有抗肿瘤作用,表明粘细菌是抗菌抗肿瘤活性物质的重要来源.Since many different types of novel compounds with biological activities are discovered form Myxobacteria, Myxobacteria become an important resource of medicine. In this experiment,through studying 95 soil and wood samples which were collected from different regions of China, 118 Myxobacteria strains were isolated in which 54 isolates were purified. The morphological identification and 16S rDNA sequencing of 54 Myxobacteria strains showed that they are distributed in 4 genera, and most of them belong to Myxococcus spp. and Sorangiurn spp.. Antibiotics and anti-tumor activities of the crude extracts of these strains were also tested.

关 键 词：粘细菌 分离 纯化 分类鉴定 活性检测 

分 类 号：Q93[生物学—微生物学]