不同地区绞股蓝总皂苷的分析及纯化  

Analysis and Purification of Total Gypenosides Sourced from Different Dreas

作　　者：秦江[1] 陈良华[1,2] 刘轩[1] 张树峰[2] 童庆宣[2] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室,福建厦门361005 [2]厦门华侨亚热带植物引种园药用植物与植物药研发中心,福建厦门361002

出　　处：《厦门大学学报（自然科学版）》2012年第1期125-129,共5页Journal of Xiamen University：Natural Science

基　　金：国家基础科学人才培养基金项目(J1030626);厦门市科技创新项目(3502Z20081143)

摘　　要：采用紫外-可见分光光度法测定产自福建、湖北、广西、云南、浙江和陕西6种不同产地绞股蓝总皂苷的含量,并通过高效液相色谱法(HPLC)比较这6种绞股蓝总皂苷组成成分的差异性.结果表明:产自湖北的绞股蓝总皂苷的含量最高,达到了11.46%,而福建绞股蓝则为9.10%.HPLC图谱结果表明产自陕西、浙江和广西地区的绞股蓝中的总皂苷成分比福建、云南和湖北地区的复杂.通过静态吸附、动态洗脱研究和比较了D101、HP-20、DM130及AB-8等4种大孔吸附树脂对绞股蓝总皂苷的富集纯化性能,发现DM130在饱和吸附和不饱和吸附两种状态条件下均对绞股蓝总皂苷的富集纯化效果最佳.以乙醇作为洗脱剂,对于动态洗脱方法最佳的乙醇体积分数为70%,对绞股蓝总皂苷的洗脱效果最好.Content and composing analyses of the total gypenosides of these six kinds of gypenoside of Gynostemma pentaphyllurn sourced from Fujian, Hubei,Guangxi, Yunnan, Zhejiang and Shanxi provinces of China＇ were made by ultraviolet-visible spectrophotometry and high performance liquid chromatography （HPLC）. It was found that the G. pentaphyllum from Hubei province got the highest content of total gypenosides of 11.46 %, while the Fujian got 9.1 %. It was showed from the HPLC fingerprints that the composing of the total gypenosides of G. pentaphyllum sourced from Shanxi, Zhejiang and Guangxi were much complex than Fujian, Yunnan and Hubei. The capability of adsorption and purification of these four kinds of macroporous resins named D101, HP-20, DM130 and AB-8 respectively were studied by means of static sorption and dynamic desorption. The result showed that DM130 got the best effect of adsorption and purification among these four in two conditions. Meanwhile, the optimum concentration of alcohol was established associated with the dynamic desorption method and it was proved that the most of gypenosides can be eluted by 70% alcohol.

关 键 词：绞股蓝总皂苷 大孔吸附树脂 高效液相色谱 

分 类 号：Q356.1[生物学—遗传学]