眼针对脑缺血再灌注损伤模型大鼠脑皮层组织IKKβ表达的影响  

Effect of eye acupunctureon on the expression of IKKβ in rat model with cerebral ischemia-reperfusion injury

作　　者：赵丹玉[1] 马贤德[1] 赵金茹[1] 王守岩[1] 王哲[1] 王德山[1] 

机构地区：[1]辽宁中医药大学基础医学院,沈阳110847

出　　处：《中华中医药杂志》2012年第1期184-186,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2007CB512700);辽宁省教育厅课题(No.L2010349)~~

摘　　要：目的:观察眼针对脑缺血再灌注损伤(CIRI)模型大鼠脑组织中IκB激酶β(IKKβ)表达,探讨眼针治疗脑损伤的作用机制。方法:健康雄性SPF级Wistar雄性大鼠60只,随机分为正常组、假手术组、模型组和眼针组。CIRI模型组和眼针组采用改良的线栓法建立大鼠大脑中动脉缺血(MACO)再灌注动物模型。RT-PCR及免疫组化方法检测大鼠缺血侧脑皮层IKKβ的mRNA和蛋白表达的变化。结果:与正常组及假手术组比较,模型组大鼠缺血侧脑皮层IKKβmRNA和蛋白水平均表达升高(P<0.01),眼针组同模型组相比,IKKβmRNA和蛋白表达则下调(P<0.01)。结论:眼针抑制脑皮层组织中IKKβ的表达,可能是眼针治疗CIRI的主要作用机制之一。Objective： To evaluate the expression of IKKβ on cerebral ischemia-reperfusion injury（CIRI） model in rat and discuss the effect of eye acupuncture therapy on brain damage.Methods： Adult male SPF Wistar rats were randomly divided into normal group,sham operation group,CIRI model group and eye acupuncture group.The model of middle cerebral artery occlusion（MACO） was established by using improved suture method.RT-PCR methods and immunohistochemical method were performed to examine the mRNA and protein expression of IKKβ in the ischemic brain cortex.Results： The mRNA and protein expression of IKKβ in CIRI model group were increased significantly（P0.01） compared with those in normal group and sham operation group,and which were decreased significantly（P0.01） in eye acupuncture group.Conclusion： eye acupuncture therapy can reduce the expression of IKKβ in CIRI,which may be part of the treatment mechanism of eye acupuncture therapy for CIRI,s.

关 键 词：眼针 脑缺血再灌注 IκB激酶β 

分 类 号：R245[医药卫生—针灸推拿学]