高效液相色谱-直流安培法测定扶本增脉颗粒中丹酚酸B的含量  

Determination of salvianolic acid B in Fuben Zengmai Granules by high performance liquid chromatography with direct current amperometric detection

作　　者：寿旦[1] 俞忠明[1] 李亚平[1] 章建民[1] 马相锋[2] 李洪玉[1] 

机构地区：[1]浙江省中医药研究院中药所,杭州310007 [2]浙江中医药大学药学院,杭州310053

出　　处：《中华中医药杂志》2012年第1期234-237,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省科技计划(No.2007F10036);浙江省级重点实验室-中药新药研发重点实验室(No.2008F3036)~~

摘　　要：目的:采用高效液相色谱-直流安培检测法建立扶本增脉颗粒中丹酚酸B的含量测定方法。方法:采用ODS-BP C18柱(200mm×4.6mm,5μm);以乙腈和0.1%磷酸(35:65,v/v)为流动相(流速1.0mL/min)进行等度洗脱;工作电极为Pt,参比电极为Ag/AgCl,工作电压为1.0V;柱温为30℃。结果:丹酚酸B质量浓度分别为0.005-10mg/L时与其峰面积呈良好的线性关系(r2=0.9998),回收率为97.8%-100.4%;相对标准偏差(RSD)<5.0%,检测限为2μg/L。结论:该方法与高效液相色谱-紫外检测法相比,含量测定结果一致,检测限更低,是一种快速、灵敏、准确的分析方法,可以为扶本增脉颗粒的质量控制提供参考。Objective： To establish a rapid and accurate method for determination of salvianolic acid B in Fubenzengmai Granules by high performance liquid chromatography coupling with direct current amperometric detection.Methods： The separation was performed on an ODS-BP C18 column（200mm×4.6mm,5μm） by gradient elution with acetonitrile and 0.1% aqueous phosphoric acid as mobile phase（35：65,v/v） at a flow rate of 1.0mL /min.The work electrode,reference electrode and work potential were Pt,Ag/AgCl,1.0 V,respectively.The temperature of column was set at 30℃.Results： Calibration curve showed good linearity and the correlation coefficients（r2） was 0.9998.Average recovery of salvianolic acid B was 97.8-100.4% and relative standard deviation（RSD） was less then 5.0%,detection limit was 2μg/L.Conclusion： The result was as same as that determined by HPLC-UV,this method has lower detection limit,and appeared to be stable,sensitive and reproducible for determination of salvianolic acid B in Fuben Zengmai Granules.The study provided a scientific basis for the quality control of Fuben Zengmai Granules.

关 键 词：高效液相色谱 安培检测 丹酚酸B 扶本增脉颗粒 

分 类 号：R286[医药卫生—中药学]