高效氯氰菊酯降解菌BCC01的分离鉴定研究  

Isolation and Identification of a Beta-cypermethrin Degrading Strain BCC01

作　　者：刘晶晶[1] 胡美英[1] 李亚楠[1] 陈少华[1] 林惠花[1] 易欣[1] 钟国华[1] 

机构地区：[1]华南农业大学昆虫毒理研究室,广东广州510642

出　　处：《安徽农业科学》2012年第1期270-272,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金(9451064201003679);国家自然科学基金(30871660)

摘　　要：[目的]分离鉴定高效氯氰菊酯降解菌。[方法]从拟除虫菊酯农药污水淤泥中,分离得到15株芽孢杆菌,通过降解HPLC测定降解活性,其中1株对高效氯氰菊酯具有较高活性,对其进行形态、生理生化16S rDNA聚类分析鉴定其种属。[结果]该菌株命名为BCC01,鉴定为蜡样芽孢杆菌,能够以高效氯氰菊酯为唯一碳源生长,4 d内对50 mg/L的高效氯氰菊酯降解率为86.8%。[结论]BCC01是对高效氯氰菊酯具有高降解活性的蜡样芽孢杆菌。[Objective] To isolate and identify a beta-cypermethrin degrading strain.[Method] 15 beta-cypermethrin（beta-CP） degrading strains were isolated from cypermethrin sewage sludge.One of them could degrade beta-CP efficiently in HPLC detection and was identified based on morphology,physiology and phylogenetic analysis of 16S rDNA gene sequence.[Result] The strain named BCC01 was identified as Bacillus cereus.In pure culture with beta-CP as sole carbon source,86.8% of beta-CP at initial concentration of 50 mg/L was degraded in 4 days by BCC01.[Conclusion] BCC01 strain belonged to Bacillus cereus with high degradation activity for beta-CP.

关 键 词：高效氯氰菊酯 生物降解 分离鉴定 

分 类 号：S131[农业科学—农业基础科学]