肝脏部分缺血再灌注对小鼠海马神经元胆碱乙酰基转移酶表达的影响  

Effect of partial hepatic ischemia/reperfusion on choline acetyltransferase expression in hippocampal neuron in mice

作　　者：邬薇薇[1] 李元海[1] 张海涅[1] 金晶星[1] 徐露[1] 

机构地区：[1]安徽医科大学第一附属医院麻醉科,合肥市230022

出　　处：《中华麻醉学杂志》2011年第11期1370-1373,共4页Chinese Journal of Anesthesiology

基　　金：安徽省教育厅重点项目（K,J2009A058Z）

摘　　要：目的探讨肝脏部分缺血再灌注对小鼠海马神经元胆碱乙酰基转移酶（ChAT）表达的影响。方法成年雄性小鼠100只，2月龄，体重20～25g，采用随机数字表法，将其随机分为5组（n=20）：正常对照组（C组）、假手术组（S组）、肝缺血20min再灌注组（I／R1组）、肝缺血30min再灌注组（I／R2组）和肝缺血40min再灌注组（I/R3组）。C组不做任何处理；S组作腹部正中切口，分离肝十二指肠韧带；I／R1组、I／R2组和I／R3组采用夹闭肝左动脉及门静脉的方法制备肝脏部分缺血再灌注损伤模型，肝缺血时间分别为20、30、40min。各组分别于术后4、18d时取10只小鼠，进行避暗实验，持续6d，然后处死，取脑组织，测定海马CA3区神经元ChAT表达。结果与C组比较，I／R1组和I／R2组术后4～7d时潜伏期缩短，错误次数增加，I／R3组术后4—9d时潜伏期缩短，错误次数增加，I／R1组、I/R2组和I／R3组术后9d时海马CA3区神经元ChAT表达下调（P〈0．05或0．01），术后18—23d时潜伏期、错误次数及术后23d时海马CA3区神经元ChAT表达差异无统计学意义（P〉0．05）；与I／R。组比较，I／R组术后4、5d时错误次数增加，I，凡组术后4—6d时潜伏期缩短，术后4～9d时错误次数增加，I/R2组和I／R3组术后9d时海马CA3区神经元ChAT表达下调（P〈0．05或0．01），术后18～23d时潜伏期、错误次数及术后23d时海马CA3区神经元ChAT表达差异无统计学意义（P〉0．05）。结论肝脏部分缺血再灌注可导致小鼠短期认知功能障碍，其机制与下调海马神经元ChAT表达有关。Objective To investigate the effects of partial hepatic ischemia/reperfusion （I/R） on choline acetyltransferase （CHAT） expression in hippocampal neuron in mice. Methods One hundred adult male mice, aged 2 months, weighing 20-25 g, were randomly divided into 5 groups （n = 20 each）： normal control group（group C）, sham operation group（group S）, groups I/R1,I/R2,I/R3. Partial hepatic ischemia was produced by clamping left hepatic artery and portal vein for 20,30,40 min respectively followed by reperfusion in groups I/R1 , I/R2, I/R3. Passive avoidance task was performed with 10 mice in each group at 4-9 and 18-23 d after operation respectively. The animals were sacrificed and their brains were removed for determination of the expression of ChAT in CA3 of hippocampal neuron. Results Compared with group C, the latency was significantly shortened and number of er- rors increased in groups I/R1 and I/R2 at 4-7 d after operation and in group I/R3 at 4-9 d after operation, the ex- pression of ChAT in hippocampal neuron down-regulated in groups I/R1 , I/R2 and I/R3 at 9 d after operation（ P 〈 0.05 or 0.01 ）. There was no significant difference in the latency and number of errors at 18-23 d after operation and the expression of ChAT in hippocampal neuron at 23 d after operation among groups C, I/R1 , I/R2 and I/R3 （ P 〉 0.05 ）. Compared with group I/R1, the number of errors was significantly increased at 4 and 5 d after operation in group I/R2 , the latency shortened at 4-6 d after operation and number of errors increased at 4-9 d after operation in group I/R3, and the expression of ChAT in hippocampal neuron down-regulated at 9 d after operation in groups I/R2 and I/R3 （ P 〈 0.05 or 0.01 ）. There was no significant difference in the latency and number of errors at 18-23 d after operation and the expresson of ChAT in hippocampal neuron at 23 d after operation among groups I/R1, I/R2 and I/R3 （P 〉 0.05）. Conclusion Partial hepatic I/R can result in transient cognitive impairment i

关 键 词：肝 再灌注损伤 胆碱O-乙酰转移酶 海马 神经元 

分 类 号：R285.5[医药卫生—中药学]