不同毒力猪繁殖与呼吸综合征病毒对仔猪肺和外周免疫器官损伤的研究  

Lesions in lung and peripheral immune organs of piglets infected with different virulence PRRSV strains

作　　者：何玉利[1] 刘永刚[1] 王刚[1] 石文达[1] 武嘉男[1] 刘鹤[1,2] 董建国[1] 韩梓峰[1,2] 姜成刚[1] 田志军[1] 蔡雪辉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物疫病诊断中心,黑龙江哈尔滨150001 [2]东北农业大学,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2012年第1期6-10,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：十二五高技术研究发展计划(2011AA10A213);哈尔滨市科技攻关计划项目(2010AA6AN 083);黑龙江省杰出青年(JC201020)

摘　　要：为研究不同毒力的PRRSV对仔猪肺脏和外周免疫器官损伤的差异,本实验分别采用PRRSV变异株(HuN4株)和PRRSV经典株(CH-1a株)感染35日龄健康的断奶仔猪,并在感染后0 d、3 d、7 d、10 d和14 d各迫杀3头,检测肺、颌下淋巴结、肠系淋巴结、腹股沟淋巴结、扁桃体和脾脏的病毒载量及病理变化情况,同时检测血清中抗PRRSV的抗体水平。结果表明:感染后3 d肺脏及各免疫器官可检测到病毒,HuN4感染组病毒载量比CH-1a感染组病毒载量高1 000倍;HuN4感染组病毒载量峰值出现在感染后10 d,而CH-1a感染组维持着较低水平的病毒载量。组织病理学检测显示HuN4感染组淋巴结内淋巴细胞显著减少,呈空泡状;CH-1a感染组淋巴结内淋巴细胞轻度减少,呈星隙状。本实验表明HuN4株比CH-1a株对肺和外周免疫器官造成更严重的损伤。To evaluate the lesions in lung and peripheral immune organs of pigs infected with different virulence PRRSV strains,piglets were inoculated with HuN4 strain and CH-1a strain,respectively.Blood samples,lung,submandibular lymph nodes（SLN）,inguinal lymph nodes（ILN）,mesenteric lymph node（MLN）,tonsil and spleen were collected at different days post inoculation（d.p.i）.The clinical signs,pathological changes,virus load in immune organs and antibodies responses were evaluated.The results showed that the virus load in lung and peripheral immune organs of the piglets infected with HuN4 strain was significantly higher in comparison with the piglets infected with CH-1a strain.Histopthologic examination showed that the lymphocytes were significantly reducedin lymph nodes of HuN4 group.These results suggest that lesions in lung and peripheral immune organs of piglets infected with HuN4 strain were more serious than that of piglets infected with CH-1a strain.

关 键 词：猪繁殖与呼吸综合征病毒 外周免疫器官 组织病理学 

分 类 号：S852.61[农业科学—基础兽医学]