犬弓首蛔虫卵黄原蛋白基因的克隆及表达分析  

Cloning and analysis of Yolk protein gene of Toxocara cains

作　　者：周荣琼[1] 马光旭[1] 周琪[1] 周作勇[1] 王芝英[1] 罗洪林[2] 夏庆友[3] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]西南大学动物科技学院,重庆北碚400716 [3]西南大学蚕学与系统生物研究所,重庆北碚400716

出　　处：《中国预防兽医学报》2012年第1期37-40,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31172313);重庆市自然科学基金(2009BB113);西南大学博士基金(11BSr04)

摘　　要：为克隆犬弓首蛔虫(T.canis)的卵黄原蛋白(YP)基因,本研究根据T.canis cDNA文库中的EST数据,采用RT-PCR方法扩增T.canis的YP基因(TcF-YP),并克隆至pGEM-T载体中进行测序。测序结果表明,TcF-YP基因片段大小为492 bp,编码163个氨基酸残基。同源分析显示,TcF-YP基因与猪蛔虫的卵黄原蛋白基因同源性最高,为78%。同时对该基因在T.canis雌虫、雄虫和雌虫各组织的表达谱进行研究,结果显示TcF-YP基因只在T.canis雌虫中高量表达,是雌虫特异的基因。对雌虫各组织的基因表达谱分析显示TcF-YP基因在子宫和肠中高量表达,在卵巢中有极微量的表达。本实验为TcF-YP基因的功能研究奠定了基础。The study was based on the EST data of cDNA library of adult of Toxocara canis.The Yolk protein gene of T.canis（TcF-YP） was amplified by RT-PCR and cloned into pGEM-T vector.The sequence analysis shown that the of TcF-YP gene was 492 bp in length encoding 163 amino acid residues which shared 78% homology with YP of Ascaris suum.The gene expression patterns of TcF-YP between female and male adult of T.canis revealed that TcF-YP was specifically expressed in female adult of T.canis.Tissue expression patterns of TcF-YP in 6 tissues of female adult of T.canis indicted that TcF-YP were highly expressed in uterus and intestine.

关 键 词：犬弓首蛔虫 卵黄原蛋白 序列分析 

分 类 号：S852.7[农业科学—基础兽医学]