磁性标记脂肪干细胞修复兔退变椎间盘  

Magnetic labeled adipose derived stem cells for repair of rabbit degenerated intervertebral disc

作　　者：陈建宇[1] 蒋新华[2] 蔡兆熙[1] 张娅[1] 刘珍珍[1] 梁碧玲[1] 

机构地区：[1]中山大学孙逸仙纪念医院放射科,广东广州510120 [2]华南肿瘤学国家重点实验室中山大学肿瘤防治中心影像与微创介入中心,广东广州510060

出　　处：《中国介入影像与治疗学》2012年第1期41-45,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：广东省科技计划项目(2008B030301318)

摘　　要：目的活体监测Gd-DTPA标记的脂肪干细胞(ADSC)在退变椎间盘内的存活、迁移和转归,观察ADSC对修复及延缓退变的作用。方法将新西兰大白兔30只制成腰椎椎间盘退变模型,2周后行Gd标记的ADSC(Gd-ADSC)移植及ADSC移植,在不同时间点摄X线片、行MR扫描及病理学检查,并与移植PBS比较。结果 Gd-ADSC移植后即刻,SPIR T1WI显示椎间盘内高信号随时间推移迅速减低。与同时间点移植PBS比较,ADSC及Gd-ADSC移植兔椎间盘退变程度较轻,髓核及纤维环内均可见胞浆丰富软骨样细胞。结论 ADSC椎间盘内移植有助于修复退变椎间盘和延缓椎间盘退变;Gd-ADSC移植后1周内可通过MRI进行监测。Objective To investigate the contribution of ADSC to repair intervertebral degenerative disc （IDD）, and to observe the in vivo tracking of transplanted Gd-DTPA labeled ADSC survive and migration by MRI. Methods Thirty Newland white rabbits underwent transplantation of Gd-DTPA labeled ADSC 2 weeks after of the establishment of IDD mod- els. X-ray films, MRI and histopathological results were obtained in time interval after transplantation of Gd-DTPA labeled ADSC and ADSC, and were compared with those of transplantation of PBS. Results High signal intensity on SPIR T1WI was detected right after intervertebral disc transplantation of Gd-DTPA labeled ADSC, but the signal intensity decreased rapidly with time going. Degeneration was mild after ADSC and Gd-DTPA labeled ADSC transplantation, and Chondrocyte-like cells was apparent in the intervertebral disc. Conclusion ADSC transplantation is effective in regeneration degenerated intervertebral disc. Gd-DTPA labeled ADSC can be identified by MRI within 1 week.

关 键 词：椎间盘退变 脂肪干细胞 磁性标记 细胞移植 

分 类 号：R681.53[医药卫生—骨科学]