重组细胞珠蛋白抗衰老与创伤愈合的初步研究  

A preliminary study of anti-aging and wound healing of recombination cytoglobin

作　　者：李招发[1,2] 赵晓芳[1] 张亭亭[1] 

机构地区：[1]华侨大学分子药物学研究所,福建泉州362021 [2]华侨大学分子药物教育部工程研究中心,福建泉州362021

出　　处：《药学学报》2012年第1期51-57,共7页Acta Pharmaceutica Sinica

基　　金：福建省自然科学基金资助项目(2010J01207);福建省重点项目资助项目(2008Y0084);华侨大学科研基金资助项目(08BS104)

摘　　要：本研究利用实验室纯化得到的重组细胞珠蛋白(rCygb),通过人永生化角质形成细胞(HaCAT)H2O2氧化应激模型、小鼠皮下连续注射D-gal导致的皮肤衰老模型、CCl4引起的大鼠急性肝损伤模型和大鼠皮肤创伤愈合模型,对rCygb抗氧化、增强机体抗氧化酶活性、降低氧自由基含量、延缓皮肤衰老以及创伤愈合作初步研究。结果表明:rCygb可以提高总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性;降低乳酸脱氢酶(LDH)和丙氨酸氨基转移酶(ALT)活性;减少丙二醛(MDA)含量。皮肤切片展示rCygb可以促进新生血管生成、增加胶原表达和提高抗炎能力。研究结果显示:rCygb可通过改善机体清除氧自由基的能力,延缓皮肤衰老和促进创伤愈合。In this paper, the preliminary study on antioxidant, enhancement of antioxidant enzymes activity, reducing the content of oxygen free radicals, delaying skin aging of the recombination cytoglobin （rCygb） purified by our lab were investigated through human keratinocyte cell line （HaCAT） H2O2 oxidative stress model, mouse skin aging model caused by continuous subcutaneous injection D-gal, rat acute liver injury model induced by CC14 and rat skin wound healing model. The results showed that rCygb improved the activities of total superoxide dismutase （T-SOD）, glutathione peroxidase （GSH-Px） and catalase （CAT）, reduced the activities of lactate dehydrogenase （LDH） and alanine aminotransferase （ALT） as well as decreased the content of malondialdehyde （MDA）. Skin biopsy showed that rCygb promoted angiogenesis, increased expression of collagen and improved the anti-inflammatory ability. All results displayed that rCygb improved the oxygen free radical scavenging ability, delayed skin aging and promoted wound healing.

关 键 词：重组细胞珠蛋白 抗氧化作用 皮肤衰老 创伤愈合 

分 类 号：R965[医药卫生—药理学]