麦胚凝集素化壳聚糖纳米粒及与N-乙酰葡萄糖胺的结合作用  

Wheat germ agglutinin anchored chitosan nanoparticles and its conjugation with N-acetylglucosamine

作　　者：李慧[1,2] 毕娟[1] 徐希明[2] 李凤前[1] 

机构地区：[1]上海市第八人民医院药剂科,上海200235 [2]江苏大学药学院,江苏镇江212013

出　　处：《药学学报》2012年第1期131-134,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30973661)

摘　　要：凝集素(lectin)是非免疫源性的糖蛋白或糖特异体(sugar-specifity),19世纪由Stillmark发现,可选择性识别细胞膜表面糖分子或受体样结构,从而与糖基化生物膜结合(溶酶体传递方式的内化过程),充当给药系统的溶酶体样"佐剂"(drug delivery adjuvant)。This study is undertaken to modify the chitosan nanoparticles （CS-NPs） with wheat germ agglutinin （WGA）, and investigate the conjugation between WGA-CS-NPs and N-acetylglucosamine （NAG）. CS-NPs were prepared by ionotropic gelation process and then conjugated with WGA under the activation of glutaricdialdehyde. The mean diameter of the CS-NPs was approximately 113.5 nm and the poly-dispersity index （PDI） was 0.18. The binding yield of WGA to CS-NPs was comprised between 27.8% and 87.9% depending mostly on the addition of 0.3% （w/v） glutaraldehyde solution. A competitive inhibition experiment of WGA-CS-NPs to bovine submaxillary gland mucin （BSM） was taken to illuminate the binding activity of WGA-CS-NPs to the sugar of N-acetylglucosamine. After the addition of NAG, the binding rates between CS-NPs and BSM almost didn＇t change, while the binding rates between WGA-CS-NPs and BSM dropped down significantly, which confirmed the specific binding characteristics of WGA to NAG.

关 键 词：壳聚糖纳米粒 麦胚凝集素 N-乙酰葡萄糖胺 牛颌下腺粘蛋白 

分 类 号：R943[医药卫生—药剂学]