经水摄入三氯乙烯对小鼠调节性T细胞的影响  

Effects of trichloroethylene intake by drinking water on regulatory T cells in mice

作　　者：王进[1] 沈彤[1,2] 徐辉[3] 许述海[3] 江涛[3] 朱启星[2,3] 

机构地区：[1]安徽医科大学公共卫生学院卫生毒理学系 [2]安徽医科大学公共卫生学院劳动卫生与环境卫生系 [3]安徽医科大学皮肤病研究所,合肥230032

出　　处：《安徽医科大学学报》2012年第1期23-26,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学重点科研项目(编号:KJ2009A73);安徽省自然科学基金项目(编号:11040606M213);教育部科学技术研究重点项目(编号:210100)

摘　　要：目的研究经水摄入三氯乙烯(TCE)对小鼠调节性T细胞(Treg)的影响。方法♀BALB/c小鼠随机分为空白对照组、溶剂对照组以及2.5、5 g/L TCE组,TCE经饮水染毒,分别在2、4、8、12周处死动物,采集外周血并取脾脏。流式细胞仪检测脾脏Treg细胞数量,荧光定量RT-PCR检测Treg细胞特异转录因子Foxp3 mRNA表达水平,ELISA法检测血清中白介素10(IL-10)含量。结果 TCE 2.5、5.0 g/L组小鼠脾脏Treg细胞数量比空白和溶剂对照组明显降低,2、4、8周时差异均有统计学意义(P<0.05,P<0.01);脾脏Foxp3 mRNA表达与对照组比较,TCE暴露组明显降低,2、4、8周时差异均有统计学意义(P<0.05,P<0.01),4周时最低;血清中IL-10含量与对照组比较,TCE 2.5、5.0 g/L组明显降低,2、4周时差异均有统计学意义(P<0.05),4周时最低;相关分析显示脾脏中Treg细胞数量和血清中细胞因子IL-10含量呈正相关(r=0.768,P<0.01)。结论经水摄入TCE对小鼠Treg细胞有显著影响。Objective To investigate the effects of trichloroethylene （TCE） intake by drinking water on regulatory T cells（Treg） in mice. Methods Female BALB/c mice were intaked 2.5 g./L and 5 g/L TCE by drinking water, water and DMSO were served as blank and vehicle control. Exposed to TCE by drinking water. At 2,4,8,12 weeks, blood were collected, spleen biopsies were taken. Treg cells number in spleen were measured by flow cytometry, Foxp3 mRNA contents in spleen were measured by Rq-PCR, IL-10 contents in serum were evaluation by ELISA. Results Treg cells were also significantly lower in TCE treated mice spleen than these in control groups, there were significant differences at 2,4,8 weeks（ P 〈 0.05 ,P 〈 0.01 ）. Foxp3 mRNA expressions in spleen of TCE treated groups also significantly decreased compared with the control groups, there were significant difference at 2, 4, 8 weeks（P 〈0.05,P 〈 0.01 ） and were minimum at 4 weeks; compared with the control group, TCE treated group IL-10 contents in serum significantly decreased, at 4 weeks minimum, 2 and 4 weeks had significant difference （ P 〈 0.05 ） ; correlation analysis showed that there were significantly positive correlation of Treg cells in spleen tissue with IL-10 contents in serum（r = 0. 768 ,P 〈 0.01 ）. Conclusion These results suggest that TCE intake by drinking water has significant effects on Treg in mice.

关 键 词：三氯乙烯 调节性T细胞 免疫毒性 

分 类 号：R114[医药卫生—卫生毒理学]