甜菜碱盐酸盐对人工瘤胃发酵参数的影响  

Effects of betaine hydrochloride on ruminal fermentation in vitro

作　　者：张善芝 韩兆玉[2] 

机构地区：[1]山东畜牧兽医职业学院,山东潍坊261061 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2012年第1期139-142,共4页Journal of Nanjing Agricultural University

基　　金：国家科技支撑计划项目(2011BAD28B02)

摘　　要：采用人工瘤胃体外发酵方法对培养液中甜菜碱盐酸盐不同质量浓度(0、16.7和33.4 mg.L-1)下,各时间段(3、6、12、18和24 h)的体外产气量、pH值、干物质降解率、氨态氮(NH3-N)、挥发性脂肪酸(VFA)、乳酸和菌体蛋白含量的测定,探讨甜菜碱盐酸盐对人工瘤胃发酵功能的影响。结果显示:培养液中甜菜碱盐酸盐的质量浓度为33.4 mg.L-1时,瘤胃液中乙酸、丙酸、丁酸、总VFA的浓度显著升高(P<0.01),干物质降解率显著提高(P<0.01),乳酸浓度显著降低(P<0.05);而甜菜碱盐酸盐对瘤胃液pH值、产气量、菌体蛋白含量、NH3-N浓度无显著影响。结论:甜菜碱盐酸盐可改善人工瘤胃发酵性能。Mixed ruminal liquid and artifial saliva were cultured in anaerobious environment for 24 h in vitro.The betaine hydrochloride was added to the media at different concentrations（0,16.7 and 33.4 mg·L-1）,and the gas production in vitro,pH value,the degradation of dry matter,NH3-N,volatile fatty acids（VFA）,lactic acid and bacteria protein productions were detected.The results showed that,when the concentration was 33.4 mg·L-1,betaine hydrochloride could increase the concentration of acetic acid,propionate acid,butyric acid and total volatile fatty acids（tVFA）（P〈0.01）,the degradation of dry matter was improved（P〈0.01）,and the concentration of lactic acid decreased（P〈0.05）.There were no notable influence（P〉0.05）adding betaine hydrochloride on pH value,gas yield,bacteria protein and NH3-N concentrations in rumen.Conclusion：the betaine hydrochloride promoted the ruminal fermentation in vitro.

关 键 词：甜菜碱盐酸盐 瘤胃发酵 体外培养 

分 类 号：S813.24[农业科学—畜牧学]