一株H9N2亚型禽流感病毒全基因组分析  

Analysis of full-length genes of a H9N2 subtype Avian influenza virus

作　　者：胡玥[1,2] 谭淑娴[1,2] 和君[1,2] 李小康[1,2] 李芳[1,2] 焦培荣[1,2] 亓文宝[1,2] 廖明[1,2] 

机构地区：[1]华南农业大学农业部动物疫病防控重点开放实验室,广州510642 [2]广东省动物源性人兽共患病预防与控制重点实验室,广州510642

出　　处：《黑龙江畜牧兽医》2012年第1期14-16,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省科技计划项目(2009B020307002;2009A0201006)

摘　　要：为了研究H9N2亚型AIV A/Chicken/Guangdong/333/2008的全基因组序列变异情况,试验利用RT-PCR方法扩增出该病毒的8个基因序列,并应用DNAStar和MEGA4.0软件分析所得基因序列。结果表明:该病毒HA基因的第226位氨基酸由Q(Gln)变为了L(Leu),其NP基因与Viet Nam/1203/2004(H5N1)的NP基因同源率最高。说明该病毒已具备了感染哺乳动物的分子特征,并可能在遗传演化过程中突变为高致病性的禽流感病毒(AIV)。To study the whole genome sequence variation of H9N2 subtype AIV A/Chicken/Guangdong/333/2008, the eight gene sequences from a H9N2 subtype AIV were amplified by RT - PCR,and analyzed by DNAStar and MEGA 4.0. The results showed that the amino acid at position 226 of HA turned Q to L,and the sequence of NP was most similar with the NP of Viet Nam/1203/2004（ H5N1 ） ,which isolated from human. The results revealed that the virus had the molecular characteristics to infect mammals,it might mutate into highly pathogenic avian in- fluenza（AIV） in the process of genetic evolution.

关 键 词：禽流感病毒 H9N2亚型 基因组 系统发生分析 

分 类 号：S852.659.5[农业科学—基础兽医学]