DM-2型大孔树脂分离纯化沙枣多糖的工艺研究  

Technical study on the separation and purification of Elaeagnus angustifolia polysaccharide by using the DM-2 macroporous resins

作　　者：李元元[1] 李炳奇[1] 刘红[1] 廉宜君[1] 马彦梅[1] 

机构地区：[1]石河子大学化学化工学院,新疆石河子832000

出　　处：《黑龙江畜牧兽医》2012年第1期110-113,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：教育部国际合作项目"春晖计划"(Z2006-1-83003);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题(BR0904)

摘　　要：为了确定DM-2型大孔树脂分离、纯化沙枣多糖的最佳工艺条件,试验以吸附率和解吸率为评价指标,通过动态试验研究各因素对分离、纯化效果的影响。结果表明:在沙枣多糖样品溶液浓度为0.7 mg/mL、pH值为10、上样速率为2.5 BV/h、上样量为3 BV、洗脱剂浓度为55%、洗脱速率为1 BV/h、洗脱剂用量为3 BV时,吸附率和解吸率分别达到84.05%和97.3%。说明在同时满足上述条件的情况下,DM-2型大孔树脂分离、纯化沙枣多糖的效率较高。To research and optimize the process conditions, a dynamic experiment was designed by setting the rates of absorption and desorption as the evaluation indexes. The result showed that the most suitable conditions contained as follows ： 0. 7 mg/mL of concentration and 10.0 of pH value for the solution of Elaeagnus angustifolia polysaccharide,2.5 BV/h of the rate, the sample loading amount of 3 BV, the eluent concentra- tion of 55%, the total quantity of eluent of 3 BV and eluent rate of 1 BV/h. The rates of absorption and desorption could achieve 84.05% and 97.3% under the conditions above. The process conditions were confirmed. It concludes that the DM -2 macroporous resins has high efficiency in the separation and purification of Elaeagnus angnstifolia polysaccharide, based on satisfying simultaneously all above conditions.

关 键 词：沙枣多糖 大孔树脂 分离 纯化 

分 类 号：R931.4[医药卫生—生药学]