Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量  

Determination of Total Phenolic Acid in the Fruits of Lycium barabarum L. by Folin-Ciocalteu Colorimetric Method

作　　者：王晓宇[1,2] 陈鸿平[1,2,3] 银玲[1] 刘友平[1,2,3] 

机构地区：[1]成都中医药大学药学院 [2]中药材标准化教育部重点实验室 [3]中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《亚太传统医药》2012年第1期45-47,共3页Asia-Pacific Traditional Medicine

基　　金：国家中医药管理局中医药行业科研专项(No.201007011)

摘　　要：目的:采用Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量。方法:以绿原酸作为对照品,以Folin-Ciocalteu试剂为显色剂,检测波长为761nm。结果:浓度为0.00318～0.0212mg/mL时与吸光度呈良好线性关系,测定方法的平均回收率为100.8%,相对标准偏差(RSD)为0.71%。结论:此法操作简便,准确可靠,为枸杞子药材的质量控制、综合开发利用提供了一定的方法和依据。Objective：The content of total Phenolic acid in the Fruits of Lyeium barabarum L. was determined by Folin-Ciocalteu Colorimetric Method. Methods：With chlorogenic acid as the reference substance, Folin Ciocalteu reagent was used as the chromogenic agent, The detection wavelength was 761nm. Results：It showed a good linear relationship between the concentration and the absorbance in the range of 0. 00318-0. 0212mg/ml,the average recovery rate of the added sample was 100.8% ,the relative standard deviation （RSD） of 0.71%. Conclusion： The Method is simple,convenient, accurate and reliable. It can be provided certain method and basis for the quality control ,comprehensive development and utilization of the Fruits of Lycium barabarum L.

关 键 词：枸杞子 Folin-Ciocalteu比色法 总酚酸 

分 类 号：R927.2[医药卫生—药学]