龟裂链霉菌工业菌中zwf1基因的阻断对土霉素生物合成影响  

Disruption of zwf1 gene encoding glucose-6-phosphate gehydrogenase enhances oxytetracycline biosynthesis in industrial Streptomyces rimosus

作　　者：郑子静[1] 于岚[1] 唐振宇[1] 郭元昕[1] 肖慈英[1] 郭美锦[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心(上海),上海200237

出　　处：《中国抗生素杂志》2012年第1期35-38,69,共5页Chinese Journal of Antibiotics

基　　金：生物反应器国家重点实验室开放课题(2060204);国家支撑项目<抗生素大规模生产关键技术创新-抗生素发酵工艺与装备集成创新研究>(2007BAI26B02)

摘　　要：目的运用代谢工程手段对龟裂链霉菌工业菌(Industrial Streptomyces rimosus,SRI)进行基因改造,提高土霉素(oxytetracycline,OTC)产量。方法利用pKC1139质粒阻断SRI基因组中葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase)编码基因zwf1。结果筛选得到一株OTC高产突变株,将突变株与原始菌株进行发酵,发现OTC产量比原始菌株提高了36.2%。结论 SRI基因组中zwf1基因的缺失使细胞合成土霉素的能力增强;龟裂链霉菌中初级代谢关键基因调控会影响次级代谢。Objective To enhance oxytetracyclin （OTC） production in Industrial Streptomyces rimosus by gene disruption according to metabolic engineering principle. Methods Disrupt zwfl gene encoding glucose-6-phosphate dehydrogenase in SRI strain using plasmid pKCl139. Results A mutant strain was successfully constructed and screened which produced OTC 36.2% more than parent strain. Conclusion The results suggest that it is an effective way to enhance OTC productivity in an industrial strain by zwfl knock-out. The regulation of key genes in primary metabolic affects secondary metabolic in S. rirnosus a lot.

关 键 词：龟裂链霉菌工业菌 葡萄糖-6-磷酸脱氢酶 基因阻断 土霉素生物合成 

分 类 号：Q756[生物学—分子生物学]