胸腺喷丁羧甲基壳聚糖鼻黏膜给药微球的制备及对大鼠CD4^+/CD8^+值的影响  

Preparation of Carboxymethyl Chitosan Microspheres Loaded with Thymopentin for Nasal Administration and the Influence on the Ratio of CD4^+/CD8^+ in Rats

作　　者：宋倩倩[1] 王选深[2] 倪玲[1] 包强[1] 李范珠[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]大连医科大学附属第二医院药剂科,辽宁大连116027

出　　处：《中国医药工业杂志》2012年第1期35-39,共5页Chinese Journal of Pharmaceuticals

基　　金：国家自然科学基金资助项目(30371781和30772793);浙江省自然科学基金杰出青年团队项目(R207722);浙江省中医药科计划重点项目(2007ZA001)

摘　　要：采用喷雾干燥法制备胸腺喷丁羧甲基壳聚糖鼻黏膜给药微球,通过正交设计优化处方,采用透析袋法研究其体外释药特性,并测定了载药微球对免疫抑制大鼠T淋巴细胞CD4+/CD8+值的影响。结果表明,所制备的微球圆整,平均粒径为(40.60±0.30)μm,包封率为(59.80±0.61)%,载药量为(15.30±0.25)%。体外释药曲线符合Higuchi方程。与阴性对照(生理盐水)组相比,大鼠鼻黏膜给予高、中、低剂量(0.43、0.30、0.23 mg/kg)载药微球后均能明显提高T淋巴细胞CD4+/CD8+值,其中高剂量载药微球组与阳性对照组(胸腺喷丁注射液,0.1 mg/kg)无显著差异(P>0.05)。The carboxymethyl chitosan microspheres loaded with thymopentin for nasal administration were prepared by spray drying technique. The preparation was optimized by orthogonal design. The drug release behavior in vitro was determined by dialysis method. The effects of thymopentin-loaded microspheres on the ratio of CD4＋/CD8＋ of T-lymphocytes in immunosuppressed rats were also examined. The optimal microspheres were spherical with the mean diameter of （40.60±0.30）~tm. The entrapment efficiency was （59.80±0.61） % and drug loading was （15.30±0.25） %. The in vitro release curve fitted to Higuchi equation. Compared with the negative control （saline） group, the CD4＋/CD8＋ ratios of high, middle and low dose （0.43, 0.30, 0.23 mg/kg） microspheres groups after nasal administration were signifi- cantly increased. There was no significant difference between high dose microspheres group and positive control （thymo- pentin injection, 0.1 mg/kg） group （P〉0.05）.

关 键 词：胸腺喷丁 羧甲基壳聚糖 微球 鼻黏膜给药 CD4+/CD8+ 

分 类 号：R944.9[医药卫生—药剂学]