戊四氮致痫大鼠海马一氧化氮的变化及对海马神经元凋亡的影响  

The changes of hippocampal NO on pentylenetetrazol induced epileptic rats and its effect to hipp-ocampal neuronal apoptosis

作　　者：唐振刚[1] 陈蕾[2] 尧青[2] 郑红花[3] 朱威[2] 李正莉[2] 

机构地区：[1]湖北医药学院附属人民医院神经外科,湖北十堰442000 [2]华中科技大学同济医学院解剖学系 [3]厦门大学医学院基础学院

出　　处：《中华实验外科杂志》2012年第1期103-104,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省卫生厅资助项目（NX2011-17）

摘　　要：目的观察戊四氮（PTZ）致痫大鼠海马一氧化氮（NO）的变化对神经元兴奋毒性的影响。方法sD雄性大鼠随机分为3组，每组14只，分别用生理盐水（A组）、PTZ（B组）、氨基胍＋PTZ（C组）造模后，对海马NO含量、谷氨酸（Glu）免疫反应性、半胱氨酸酶（Caspase）-3mRNA水平进行检测分析。结果B组海马NO含量为（75．67±2．04）μmol／L，与A组（11．90±0．64）μmol／L和c组（36．19±4．48）μmol／L比较差异有统计学意义（P〈0．05）；A组和c组的Glu、caspase-3mRNA表达水平明显低于B组（P〈0．05）。结论戊四氮致痫后可导致大鼠海马NO过量产生，对海马神经元具有兴奋毒性作用。而一氧化氮合成酶抑制剂的干预，具有神经保护作用。Objective To study the changes of nitric oxide （NO） content in hippocampus of rat by Pentylenetetrazol （PTZ） -induced seizures models and its effect of toxicity and apoptosis. Methods SD male rats were random divided into three groups, each group has 14 rats. Group A is the saline control group, group B is the pentylenetetrazol induced epileptic group and group C is aminoguanidine （NOS inhibitor） pretreatment plus pentylenetetrazol induced epileptic group. NO content in rat hippocampus was detected by ultraviolet spectrophotometry, glutamate （Glu） immunoreactivity in rat hippocampus was detected by immunohistochemistry （SABC） and caspase-3 mRNA levels in rat hippocampus were detected by reverse transcription-polymerase chain reaction （RT-PCR） respectively at 2, 48 h after modeling. Results Group A had no seizure, group B had epilepsia gravior over V grade, rats in group C had seizure over Ⅱ - Ⅲ grade： The NO content on group B （75.67±2. 04） μmol/L was significantly higher than group A （ 11.90±0. 64） μmol/L and group C （36. 19±4.48 ） μmol/L （ P 〈 0. 05 ） ; Glu immunoreactivity and caspase-3 mRNA leves of group A and group C were lower than group B （P 〈 0. 05）. Conclusion PTZ-induced seizures may lead to excessive production of NO in hippocampus, which had excited toxicity to hippocampal neurons. The intervention of NOS synthetic inhibitor had neuroprotective effects.

关 键 词：癫痫 一氧化氮 谷氨酸 半胱氨酸酶-3 凋亡 

分 类 号：R285.5[医药卫生—中药学]