关苍术超临界提取物对乙醇致大鼠胃黏膜损伤保护作用的研究  

Protective Effects of Supercritical CO_2 Extract from Atractylodes japonica on Ethanol-induced Gastric Mucosal Injury in Rats

作　　者：刘男[1] 廉丽花[1] 张颖丽[1] 张善玉[1] 

机构地区：[1]长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《中国药房》2012年第3期215-217,共3页China Pharmacy

摘　　要：目的:研究关苍术超临界提取物(GCT)对乙醇致大鼠胃黏膜损伤的保护作用。方法:40只Wistar大鼠均分成5组,即空白对照(等容蒸馏水)、模型(等容蒸馏水)、铝碳酸镁(0.37g.kg-1)和GCT高、低剂量(0.70、0.35g.kg-1)组。ig给药,每天1次,连续5d,末次给药30min后ig无水乙醇(0.5mL.100g-1)复制急性胃黏膜损伤模型,观察胃黏膜损伤面积,测定胃组织中丙二醛(MDA)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)、一氧化氮合成酶(NOS)活性及环氧化物酶2(COX-2)蛋白表达水平。结果:高、低剂量GCT能显著抑制乙醇引起的大鼠胃黏膜损伤及胃组织中MDA含量的增加和MPO活性的升高,抑制SOD活性的减弱、NO含量和NOS活性的降低,并抑制COX-2蛋白的表达。结论:GCT能拮抗乙醇引起的胃黏膜损伤,其机制可能与通过抑制氧自由基产生、维持胃黏膜中NO正常水平、下调COX-2表达有关。OBJECTIVE： To study the protective effects of supercritical CO2 extract from Atractylodes japonica（GCT）against ethanol-induced gastric mucosal injury in rats. METHODS： 40 Wistar rats were divided into 5 groups, i.e. normal control group （constant volume distilled water）, model group （constant volume distilled water）, hydrotalcite group （0.37 g. kg^-1） , GCT high-dose and low-dose groups （0.70,0.35 g.kgl）.The rat model of acute gastric damage was induced by absolute ethanol （0.5 mL. 100 g^-1） intragastrically. Thgse groups were given relevant medicine intragastrically once a day for 5 days. Model was induced 30 min after the last medication. The gastric mucosal injury area was observed. The contents of MDA and NO, the activity of SOD, MPO and NOS, and the expression of COX-2 in gastric mucous were determined. RESULTS： High-dose and low-dose of GCT sup- pressed the elevation of MDA and MPO, and enhanced the SOD activity, the decrease of NO content and NOS activity. Moreover, GCT significantly reduced COX-2 expression. CONCLUSION： GCT can prevent ethanol-induced gastric mucosal injury, partly through scavenging free radical, maintaining NO level and down-regulating COX-2.

关 键 词：关苍术 乙醇 胃黏膜损伤 环氧化物酶2 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]