GC法测定藿香中甲基胡椒酚的含量  

Study on Determination of Estragole in Agastache rugosa by GC

作　　者：薛雯[1,2] 孙晖[2] 薛健[1] 程猷[2] 吴晓波[1] 庞作正[2] 

机构地区：[1]中国医学科学院/北京协和医学院药用植物研究所分析室,北京100193 [2]黑龙江中医药大学药学院药物分析教研室,哈尔滨150040

出　　处：《中国药房》2012年第3期230-231,共2页China Pharmacy

基　　金：国家科技重大专项课题(2009ZX09502-027);国家科技重大专项子课题(2009ZX09308-001-1-5)

摘　　要：目的:建立测定藿香中甲基胡椒酚含量的方法。方法:采用气相色谱法。色谱柱为DB-5石英毛细管柱(30m×0.32mm×0.25μm),进样口温度为230℃,检测器温度为300℃,流速为1.0mL.min-1,载气为氮气,不分流进样,进样量为1μL。以外标法定量。结果:甲基胡椒酚的检测浓度在0.02～2.00g.L-1范围内与峰面积积分值呈良好线性关系(r=0.9993);平均回收率为103.93%,RSD=2.1%(n=6)。结论:该方法简便、准确、快速,可用于测定藿香中甲基胡椒酚的含量。OBJECTIVE： To establish a method for the content determination of estragole in Agastache rugosa. METHODS： The contents of estragole was determined by GC with DB-5 fused capillary （30 m×0.32 mm×0.25μm） column at the flow rate of 1.0 mL. min^-1.The temperature of injector and detector were 230 ℃ and 300 ℃. Nitrogen was used as carrier gas with splitless inlet and injection volume was 1 μL using external standard method. RESULTS： The linear ranges of estragole was 0.02-2.00 g. L^-1（r=0.999 3）with an average recovery of 103.93% （RSD=2.1%, n=6）. CONCLUSION： The method is rapid, simple and accu- rate for the content determination of estragle in A. rugosa.

关 键 词：气相色谱法 藿香 甲基胡椒酚 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]