不同产地丹参及丹参配伍黄芪前后有效成分含量测定  

Content Determination of Effective Ingredients of Salvia miltiorrhiza before and after Mixed with Astragalus membranceus and Samples from Different Producing Areas

作　　者：陈燕芬[1] 王磊[1] 陈丽娟[1] 钟军[1] 曾祥就[1] 

机构地区：[1]广东省中医院,广州510120

出　　处：《中国药房》2012年第3期237-239,共3页China Pharmacy

基　　金：广东省中医药局科研课题(2008123)

摘　　要：目的:测定不同产地丹参药材中丹参酮ⅡA与丹酚酸B的含量,考察丹参与黄芪配伍前后丹参中有效成分的含量变化情况。方法:采用高效液相色谱法对丹参酮ⅡA与丹酚酸B进行含量测定。测定丹参酮ⅡA的色谱柱为Waters C18(250 mm×4.6 mm,5μm),流动相为甲醇-水(75∶25),检测波长为270 nm;测定丹酚酸B的色谱柱为Agilent C18(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-甲酸-水(30∶10∶1∶59),检测波长为286 nm。结果:不同产地丹参中丹参酮ⅡA的含量大多数低于药典规定,丹酚酸B的含量有高有低;丹参配伍黄芪后可提高丹参中丹参酮ⅡA和丹酚酸B的提取率。结论:不同产地丹参中丹参酮ⅡA和丹酚酸B的含量无相关性,其含量差异说明丹参的栽培技术有待加强;丹参配伍黄芪后有效成分含量的变化说明了二者配伍具有科学性与合理性。OBJECTIVE： To determine the contents of tanshinone ⅡA and salvianolic acid B in Salvia miltiorrhiza from different producing areas, and to investigate the changes of different components of S. miltiorrhiza before and after mixed with Astragalus membranceus. METHODS： HPLC method was used to measure the content of tanshinone ⅡA and salvianolic acid B. The determination of tanshinone ⅡA was performed on Waters C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of methanol-water （75：25） at detection wavelength of 270 nm. The determination of salvianolic acid B was carried on Agilent C18（250 mm× 4.6 mm, 5 μm） column with mobile phase are used methanol-acetonitrile-formic acid-water （30 ： 10 ： 1 ： 59） at the detection wavelength of 286 nm. RESULTS： The contents of tanshinone II ~ in S. miltiorrhiza from different producing areas were lower than the standard stated in pharmacopeia, and salvianolic acid B had a large fluctuation. The extraction rate of tanshinone ⅡA and salvianolic acid B could be improved after mixed with A. membranceus. CONCLUSION： The contents of tanshinone ⅡA and salvianolic acid B from different origin places do not exist correlation. The cultivation technology of S. miltiorrhiza needs to be improved. It shows science and rationality that the contents of effective components in S. miltiorrhiza changes after mixed with A. membranceus.

关 键 词：高效液相色谱法 丹参 产地 配伍 黄芪 丹参酮ⅡA 丹酚酸B 含量 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]