HPLC法同时测定妇可靖胶囊中木犀草素和芍药苷的含量  

Simultaneous Determination of Luteolin and Paeoniflorin in Fukejing Capsule by HPLC

作　　者：袁春玲[1] 王佩琪[1] 郭伟英[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《中国药房》2012年第3期263-265,共3页China Pharmacy

摘　　要：目的:建立同时测定妇可靖胶囊中木犀草素和芍药苷含量的方法。方法:色谱柱为Chromatrex C18(200 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸(35∶65),检测波长为230 nm(检测芍药苷)和350 nm(检测木犀草素),流速为1.0 mL·min-1。结果:木犀草素、芍药苷的进样量分别在0.065～0.325μg(r=0.999 9)、0.05～0.25μg(r=0.999 8)范围内与各自峰面积积分值呈良好线性关系;平均加样回收率分别为99.78%、100.08%,RSD分别为0.31%、0.62%(n=9)。结论:本方法简便、准确,可用于妇可靖胶囊的质量控制。OBJECTIVE： To establish a method for the simultaneous determination of luteolin and paeoniflorin in Fukejing capsule. METHODS： The Chromatrex CI8 （200 minx4.6 mm, 5 μm） column was used with the mobile phase consisted of methanol-0.4% phosphoric acid （35：65） at the flow rate of 1.0 rnL.min-1. The detection wavelength were 230 nm for paeoniflorin and 350 nm for luteolin. RESULTS： The linear range of luteolin and paeoniflorin were 0.065-.325 μg（r=0.999 9）and 0.05-0.25μg （r=0.999 8）, respectively. The average recovery rates were 99.78% （RSD=0.31%, n=9）, 100.08% （RSD=0.62%, n=9）,respectively. CONCLUSION： The method is simple, accurate and can be used to quality control of Fukejing capsule.

关 键 词：高效液相色谱法 妇可靖胶囊 木犀草素 芍药苷 含量测定 

分 类 号：R283.65[医药卫生—中药学] R927.2[医药卫生—中医学]